Inhibition by berberine of cyclooxygenase-2 transcriptional activity in human colon cancer cells

被引：270

作者：

Fukuda, K

Hibiya, Y

Mutoh, M

Koshiji, M

Akao, S

Fujiwara, H

机构：

[1] Gifu Univ, Sch Med, Dept Oriental Med, Gifu 5008705, Japan

[2] Natl Canc Ctr, Res Inst, Canc Prevent Div, Chuo Ku, Tokyo 104, Japan

[3] Gifu Univ, Sch Med, Dept Internal Med 2, Gifu 5008705, Japan

来源：

JOURNAL OF ETHNOPHARMACOLOGY | 1999年 / 66卷 / 02期

关键词：

berberine; cyclooxygenase-2; colon cancer; transcriptional activity;

D O I：

10.1016/S0378-8741(98)00162-7

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The enzyme cyclooxpgenase-2 (COX-2) is abundantly expressed in colon cancer dens and plays a key role in colon tumorigenesis. Compounds inhibiting COX-2 transcriptional activity have therefore potentially a chemopreventive property against colon tumor formation. An assay method for estimating COX-2 transcriptional activity in human colon cancer cells was established using a beta-galactosidase reporter gene system, and examination was made of various medicinal herbs and their ingredients for an inhibitory effect on COX-2 transcriptional activity. We found that berberine, an isoquinoline alkaloid present in plants of the genera Berberis and Coptis, effectively inhibits COX-2 transcriptional activity in colon cancer cells in a dose- and time-dependent manner at concentrations higher than 0.3 mu M. The present findings may further explain the mechanism of anti-inflammatory and anti-tumor promoting effects of berberine. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：227 / 233

页数：7

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