Pharmacological and molecular characterization of 5-hydroxytryptamine7 receptors in the rat adrenal gland

被引：39

作者：

Contesse, V ^{[1
]}

Lenglet, S ^{[1
]}

Grumolato, L ^{[1
]}

Anouar, Y ^{[1
]}

Lihrmann, I ^{[1
]}

Lefebvre, H ^{[1
]}

Delarue, C ^{[1
]}

Vaudry, H ^{[1
]}

机构：

[1] Univ Rouen, Unite Associee Ctr Natl Rech Sci, INSERM,European Inst Peptide Res,IFRMP 23, U413,Lab Cellular & Mol Neuroendocrinol, F-76821 Mont St Aignan, France

来源：

MOLECULAR PHARMACOLOGY | 1999年 / 56卷 / 03期

关键词：

D O I：

10.1124/mol.56.3.552

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Serotonin (5-hydroxytryptamine; 5-HT) is a potent stimulator of aldosterone secretion in the rat adrenal gland but the type of receptor involved in the mechanism of action of 5-HT remains unknown. The aim of the present study was to determine the pharmacological profile and to clone the receptor responsible for the corticotropic effect of 5-HT in rat glomerulosa cells. A series of 10 serotonergic receptor agonists and 12 receptor antagonists was used to characterize the receptor mediating the effect of 5-HT on aldosterone secretion from perifused rat adrenocortical slices. Correlation analysis between the potencies of the different compounds in our model and those previously reported for various recombinant 5-HT receptors showed that the rat adrenal 5-HT receptor exhibits the same pharmacological profile as the 5-HT, receptor transiently expressed in COS-7 cells (r = 0.82 for agonists, p < .05; r = 0.83 for antagonists, p < .01). Polymerase chain reaction with specific primers revealed the expression of 5-HT7 receptor mRNA in the rat adrenal gland. Cloning of the polymerase chain reaction product confirmed that the amplified DNA corresponded to the 5-HT7 receptor cDNA sequence. Western blot analysis showed the presence of a protein with an apparent molecular mass of 66 kDa in the adrenal cortex but not in the medulla. Taken together, these data demonstrate that the rat adrenal glomerulosa expresses functional 5-HT7 receptors. Rat glomerulosa cells will thus provide a robust and sensitive bioassay for future studies on native 5-HT7 receptors.

引用

页码：552 / 561

页数：10

共 47 条

[1] CLONING OF ANOTHER HUMAN SEROTONIN RECEPTOR (5-HT1F) - A 5TH 5-HT1 RECEPTOR SUBTYPE COUPLED TO THE INHIBITION OF ADENYLATE-CYCLASE [J].