Low temperature and aging-promoted expression of PUMP in potato tuber mitochondria

被引：50

作者：

Nantes, IL

Fagian, MM

Catisti, R

Arruda, P

Maia, IG

Vercesi, AE ^{[1
]}

机构：

[1] Univ Estadual Campinas, Fac Ciencias Med, Dept Patol Clin, NMCE, BR-13083970 Campinas, SP, Brazil

[2] Univ Estadual Campinas, Ctr Biol Mol & Engn Genet, BR-13083970 Campinas, SP, Brazil

来源：

FEBS LETTERS | 1999年 / 457卷 / 01期

基金：

巴西圣保罗研究基金会;

关键词：

plant uncoupling mitochondrial protein; thermogenesis; potato tuber mitochondrion; reactive oxygen;

D O I：

10.1016/S0014-5793(99)01017-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this communication, we show that the plant uncoupling mitochondrial protein (PUMP) present in potato tuber mitochondria is induced by aging at 28 degrees C and that this induction is strongly stimulated when the potato tubers are stored at low temperature (4 degrees C). PUMP activity was detected by the degree of linoleic acid (LA)-induced ATP-sensitive mitochondrial uncoupling measured as a function of the decrease in membrane potential(Delta Psi). The PUMP content was evaluated by immunoblot analysis using polyclonal antibodies raised against potato PUMP that specifically detected a 32 kDa band. In agreement with the effect of LA on AY, the content of the 32 kDa band increased during storage and was stimulated by low temperature. These results support the proposed role of PUMP in plant thermogenesis and possibly in fruit ripening and senescence. (C) 1999 Federation of European Biochemical Societies.

引用

页码：103 / 106

页数：4

共 23 条

[1] Cyanide-resistant, ATP-synthesis-sustained, and uncoupling-protein-sustained respiration during postharvest ripening of tomato fruit [J].