In vitro evaluation of poly[bis(ethyl alanato)phosphazene] as a scaffold for bone tissue engineering

被引：15

作者：

Conconi, Maria Teresa

Lora, Silvano

Menti, Anna Michela

Carampin, Paolo

Parnigotto, Pier Paolo

机构：

[1] Univ Padua, Dept Pharmaceut Sci, I-35131 Padua, Italy

[2] CNR, Inst Organ Synthesis & Photoreact, Legnaro, Italy

来源：

TISSUE ENGINEERING | 2006年 / 12卷 / 04期

关键词：

D O I：

10.1089/ten.2006.12.811

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Polyphosphazenes with amino acid ester as side groups are biocompatible polymers that could provide valid scaffolds for cell growth. In the present study we investigate the adhesion and growth of osteoblasts obtained from rat bone marrow on matrices composed of thin fibers of poly[bis(ethyl alanato) phosphazene] (PAlaP), poly(d,l-lactic acid) (PDLLA), or PAlaP/PDLLA blend. Our data show that scaffolds of PAlaP or PAlaP/PDLLA blend enhanced the cell adhesion and growth in comparison with that observed in cultures seeded on polystyrene tissue culture plates. Although collagenase-digestible protein synthesis remained unchanged, all scaffolds induced a decrease in alkaline phosphatase activity, suggesting that osteoblasts are in the proliferation phase. Both PAlaP and PAlaP blended with PDLLA may represent a new and interesting substrate for bone tissue engineering.

引用

页码：811 / 819

页数：9

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