Simultaneous analysis of prostanoids using liquid chromatography/high-field asymmetric waveform ion mobility spectrometry/tandem mass spectrometry

被引：39

作者：

Kapron, James

Wu, Jin

Mauriala, Timo

Clark, Patsy

Purves, Randy W.

Bateman, Kevin P.

机构：

[1] Merck Frosst Ctr Therapeut Res, Kirkland, PQ H9H 3L1, Canada

[2] Ionalytics, Ottawa, ON K1A 0R6, Canada

来源：

RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 2006年 / 20卷 / 10期

关键词：

D O I：

10.1002/rcm.2505

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A liquid chromatography/high-field asymmetric waveform ion mobility spectrometry/tandem mass spectrometry (LC-FAIMS-MS/MS) semi-quantitative method was developed for the simultaneous determination of prostaglandin (PG) E-2, PGD(2), PGF(2 alpha), 6-keto-PGF(1 alpha), and thromboxane (TX) B-2. Diluted samples containing these prostanoids and their tetra-deuterium-substituted internal standards were analyzed by LC followed by either selected reaction monitoring (LC-SRM) or FAIMS and selected reaction monitoring (LC-FRM). FAIMS reduced background noise, separated the isobaric ions PGE(2) and PGD(2), and separated dynamically interchanging TXB2 anomers. This is the first report of the separation of interconverting anomers by FAIMS. Generally, the LC-FRM chromatograms were more selective than the LC-SRM chromatograms. Its potential was demonstrated by analysis of prostanoids in guinea pig lumbar spinal cord homogenate. Copyright (c) 2006 John Wiley & Sons, Ltd.

引用

收藏

页码：1504 / 1510

页数：7

相关论文

共 26 条

[1] Gas Chromatographic Determination of Prostaglandins [J].

Baranowski, R. ;

Pacha, K. .

MINI-REVIEWS IN MEDICINAL CHEMISTRY, 2002, 2 (02) :135-144

[2] Separation of β2-microglobulin conformers by high-field asymmetric waveform ion mobility spectrometry (FAIMS) coupled to electrospray ionisation mass spectrometry [J].

Borysik, AJH ;

Read, P ;

Little, DR ;

Bateman, RH ;

Radford, SE ;

Ashcroft, AE .

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2004, 18 (19) :2229-2234

[3] Deletion of microsomal prostaglandin E2 (PGE2) synthase-1 reduces inducible and basal PGE2 production and alters the gastric prostanoid profile [J].

Boulet, L ;

Ouellet, M ;

Bateman, KP ;

Ethier, D ;

Percival, MD ;

Riendeau, D ;

Mancini, JA ;

Méthot, N .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (22) :23229-23237

[4] FORMATION OF ISOINDOLE DERIVATIVES OF SULFIDOPEPTIDE LEUKOTRIENES BY REACTION WITH ORTHO-PHTHALALDEHYDE AND SEPARATION BY REVERSE PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].

DOBROWSKY, RT ;

OSULLIVAN, G ;

BALLAS, LM ;

FLEISHER, LN ;

OLSON, NC .

JOURNAL OF LIQUID CHROMATOGRAPHY, 1987, 10 (01) :137-160

[5] Rapid and sensitive differentiation of anomers, linkage, and position isomers of disaccharides using High-Field Asymmetric Waveform Ion Mobility Spectrometry (FAIMS) [J].

Gabryelski, W ;

Froese, KL .

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 2003, 14 (03) :265-277

[6] Carrageenan-induced paw edema in rat elicits a predominant prostaglandin E2 (PGE2) response in the central nervous system associated with the induction of microsomal PGE2 synthase-1 [J].

Guay, J ;

Bateman, K ;

Gordon, R ;

Mancini, J ;

Riendeau, D .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (23) :24866-24872

[7] High-field asymmetric waveform ion mobility spectrometry: A new tool for mass spectrometry [J].

Guevremont, R .

JOURNAL OF CHROMATOGRAPHY A, 2004, 1058 (1-2) :3-19

[8]

He JL, 1998, TALANTA, V46, P1

[9] Analysis of prostaglandins by micellar electrokinetic capillary chromatography [J].

Hsieh, YZ ;

Kuo, KL .

JOURNAL OF CHROMATOGRAPHY A, 1997, 761 (1-2) :277-284

[10] Removal of metabolite interference during liquid chromatography/tandem mass spectrometry using high-field asymmetric waveform ion mobility spectrometry [J].

Kapron, JT ;

Jemal, M ;

Duncan, G ;

Kolakowski, B ;

Purves, R .

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2005, 19 (14) :1979-1983