A BioBrick compatible strategy for genetic modification of plants

被引：16

作者：

Boyle, Patrick M. ^{[1
]}

Burrill, Devin R. ^{[1
]}

Inniss, Mara C. ^{[1
]}

Agapakis, Christina M. ^{[1
]}

Deardon, Aaron ^{[2
]}

DeWerd, Jonathan G. ^{[2
]}

Gedeon, Michael A. ^{[2
]}

Quinn, Jacqueline Y. ^{[2
]}

Paull, Morgan L. ^{[2
]}

Raman, Anugraha M. ^{[2
]}

Theilmann, Mark R. ^{[2
]}

Wang, Lu ^{[2
]}

Winn, Julia C. ^{[2
]}

Medvedik, Oliver ^{[3
]}

Schellenberg, Kurt ^{[4
]}

Haynes, Karmella A. ^{[1
]}

Viel, Alain ^{[3
]}

Brenner, Tamara J. ^{[3
]}

Church, George M. ^{[5
,6
]}

Shah, Jagesh V. ^{[1
]}

Silver, Pamela A. ^{[1
,5
]}

机构：

[1] Harvard Univ, Sch Med, Dept Syst Biol, Boston, MA 02115 USA

[2] Harvard Univ, Harvard Coll, Cambridge, MA 02138 USA

[3] Harvard Univ, Dept Mol & Cellular Biol, Cambridge, MA 02138 USA

[4] Harvard Univ, Arnold Arboretum, Boston, MA 02131 USA

[5] Harvard Univ, Wyss Inst Biol Inspired Engn, Boston, MA 02115 USA

[6] Harvard Univ, Sch Med, Dept Genet, Boston, MA 02115 USA

来源：

JOURNAL OF BIOLOGICAL ENGINEERING | 2012年 / 6卷 / 01期

基金：

美国国家科学基金会;

关键词：

iGEM; Synthetic biology; Arabidopsis; Plant biotechnology; TASTE-MODIFYING PROTEIN; FUNCTIONAL EXPRESSION; MIRACULIN; BRAZZEIN; CELLS; DNA;

D O I：

10.1186/1754-1611-6-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Background: Plant biotechnology can be leveraged to produce food, fuel, medicine, and materials. Standardized methods advocated by the synthetic biology community can accelerate the plant design cycle, ultimately making plant engineering more widely accessible to bioengineers who can contribute diverse creative input to the design process. Results: This paper presents work done largely by undergraduate students participating in the 2010 International Genetically Engineered Machines (iGEM) competition. Described here is a framework for engineering the model plant Arabidopsis thaliana with standardized, BioBrick compatible vectors and parts available through the Registry of Standard Biological Parts (www. partsregistry. org). This system was used to engineer a proof-of-concept plant that exogenously expresses the taste-inverting protein miraculin. Conclusions: Our work is intended to encourage future iGEM teams and other synthetic biologists to use plants as a genetic chassis. Our workflow simplifies the use of standardized parts in plant systems, allowing the construction and expression of heterologous genes in plants within the timeframe allotted for typical iGEM projects.

引用

页数：7

共 30 条

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