Bead-based suspension array for simultaneous detection of antibodies against the Rift Valley fever virus nucleocapsid and Gn glycoprotein

被引：25

作者：

van der Wal, Fimme J. ^{[1
]}

Achterberg, Rene P. ^{[1
]}

de Boer, S. Matthijn ^{[3
]}

Boshra, Hani ^{[4
]}

Brun, Alejandro ^{[4
]}

Maassen, Catharina B. M. ^{[1
]}

Kortekaas, Jeroen ^{[2
]}

机构：

[1] Wageningen Univ, Res Ctr, Cent Vet Inst, Dept Infect Biol, NL-8200 AB Lelystad, Netherlands

[2] Wageningen Univ, Res Ctr, Cent Vet Inst, Dept Virol, NL-8200 AB Lelystad, Netherlands

[3] Univ Utrecht, Fac Vet Med, Div Virol, Dept Infect Dis & Immunol, NL-3584 CL Utrecht, Netherlands

[4] Ctr Invest Sanidad Anim CISA INIA, Madrid 28130, Spain

来源：

JOURNAL OF VIROLOGICAL METHODS | 2012年 / 183卷 / 02期

关键词：

Rift Valley fever virus; Nucleocapsid; Glycoprotein; Serology; Bead-based suspension array; Luminex; DIVA; LINKED-IMMUNOSORBENT-ASSAY; IGM ANTIBODIES; INDIRECT ELISA; CAPTURE ELISA; NSS PROTEIN; N-PROTEIN; VALIDATION; SANDWICH; DISEASE; HUMANS;

D O I：

10.1016/j.jviromet.2012.03.008

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A multiplex bead-based suspension array was developed that can be used for the simultaneous detection of antibodies against the surface glycoprotein Gn and the nucleocapsid protein N of Rift Valley fever virus (RVFV) in various animal species. The N protein and the purified ectodomain of the Gn protein were covalently linked to paramagnetic Luminex beads. The performance of the resulting multiplex immunoassay was evaluated by testing a comprehensive and well-characterized panel of sera from sheep, cattle and humans. The suitability of this multiplex immunoassay to differentiate infected from vaccinated animals (DIVA) was investigated by testing sera from lambs vaccinated with a paramyxovirus vaccine vector expressing the RVFV surface glycoproteins Gn and Cc. The results suggest that the bead-based suspension array can be used as a DIVA assay to accompany several recently developed experimental vaccines that are based on RVFV glycoproteins, and are devoid of the N protein. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：99 / 105

页数：7

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