Impact of intestinal microbiota and gastrointestinal conditions on the in vitro survival and growth of Bacillus cereus

被引：22

作者：

Ceuppens, Siele ^{[1
,2
]}

Van de Wiele, Tom ^{[1
]}

Rajkovic, Andreja ^{[2
]}

Ferrer-Cabaceran, Tomas ^{[2
]}

Heyndrickx, Marc ^{[3
,4
]}

Boon, Nico ^{[1
]}

Uyttendaele, Mieke ^{[2
]}

机构：

[1] Univ Ghent, Fac Biosci Engn, Lab Microbial Ecol & Technol LabMET, Dept Biochem & Microbial Technol, B-9000 Ghent, Belgium

[2] Univ Ghent, Fac Biosci Engn, Dept Food Safety & Food Qual, LFMFP, B-9000 Ghent, Belgium

[3] Inst Agr & Fisheries Res ILVO, Technol & Food Sci Unit, Minist Flemish Community, Melle, Belgium

[4] Univ Ghent, Fac Vet Sci, Dept Pathol Bacteriol & Poultry Dis, Merelbeke, Belgium

来源：

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY | 2012年 / 155卷 / 03期

关键词：

Bacillus cereus NVH 1230-88; In vitro simulation; Gastrointestinal passage; Competitive exclusion; Intestinal microbiota; VEGETATIVE CELLS; ENTEROTOXIN PRODUCTION; SPORES; QUANTIFICATION; VALIDATION; SIMULATOR; REACTOR; MODEL;

D O I：

10.1016/j.ijfoodmicro.2012.02.013

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Ingestion of B. cereus can result in diarrhea, if these bacteria survive gastrointestinal passage and achieve growth and enterotoxin production in the small intestine. The gastrointestinal survival of vegetative cells and spores of the diarrheal food poisoning strain B. cereus NVH 1230-88 was investigated during in vitro batch experiments simulating the stomach, duodenum and ileum using simulation media and competing intestinal microbiota. All spores and approx. 30% of the vegetative B. cereus cells survived the 2 h incubation in gastric medium with pH 4.0. Sterile intestinal medium induced germination of spores and enabled outgrowth of vegetative cells to approx. 7 log CFU/mL. The behavior of B. cereus in the intestinal environment with competing intestinal bacteria was determined by their relative concentrations. Besides the numbers of intestinal bacteria, the nutrition and composition of the intestinal community were also very important for the growth inhibition of B. cereus. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：241 / 246

页数：6

共 25 条

[1] STRUCTURAL AND FUNCTIONAL-ANALYSIS OF THE PROMOTER REGION INVOLVED IN FULL EXPRESSION OF THE CRYIIIA TOXIN GENE OF BACILLUS-THURINGIENSIS [J].