Messenger RNAs in synaptosomal fractions from rat brain

被引：12

作者：

Crispino, M

Capano, CP

Aiello, A

Iannetti, E

Cupello, A

Giuditta, A ^{[1
]}

机构：

[1] Univ Naples Federico II, Dipartimento Fisiol Gen & Ambientale, Naples, Italy

[2] Univ Catanzaro Magna Grecia, Fac Farm, I-88021 Roccelletta Di Borgia, CZ, Italy

[3] CNR, Ist Int Genet & Biofis, I-80125 Naples, Italy

[4] CNR, Ctr Neurofisiol Cerebrale, Genoa, Italy

来源：

MOLECULAR BRAIN RESEARCH | 2001年 / 97卷 / 02期

关键词：

synaptosome; nerve terminal; presynaptic ending; mRNA; RT-PCR;

D O I：

10.1016/S0169-328X(01)00321-7

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Synaptosomal fractions from rat brain have been analyzed with semi-quantitative RT-PCR methods to determine their content of mRNAs coding for presynaptic, postsynaptic, glial, and neuronal proteins. Each mRNA was determined with reference to the standard HPRT mRNA. In our analyses, mRNAs were considered to be associated with synaptosomes only if their relative amounts were higher than in microsomes prepared in a polysome stabilizing medium, rich in Mg++ and K+ ions, or in the homogenate. According to this stringent criterion, the following synaptosomal mRNAs could not be attributed to microsomal contamination and were assumed to derive from the subcellular structures known to harbor their translation products, i.e. GAT-1 mRNAs from presynaptic terminals and glial processes, MAP2 mRNA from dendrites, GFAP mRNA from glial processes, and TAU mRNA from neuronal fragments. This interpretation is in agreement with the involvement of extrasomatic mRNAs in local translation processes. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：171 / 176

页数：6

共 34 条

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