A composite map of expressed sequences in maize

被引：80

作者：

Causse, M ^{[1
]}

Santoni, S ^{[1
]}

Damerval, C ^{[1
]}

Maurice, A ^{[1
]}

Charcosset, A ^{[1
]}

Deatrick, J ^{[1
]}

deVienne, D ^{[1
]}

机构：

[1] UNIV PARIS SUD,INRA,CNRS,STN GENET VEGETALE,URA 1492,INST NATL AGRON PARIS GRIGNON,F-91190 GIF SUR YVETTE,FRANCE

来源：

GENOME | 1996年 / 39卷 / 02期

关键词：

Zea mays L; RFLP genetic map; molecular markers; proteins;

D O I：

10.1139/g96-053

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A maize genetic map based mainly on expressed sequences has been constructed. The map incorporates data from four segregating populations. Three recombinant inbred line populations were derived from the nonreciprocal crosses between three inbred lines. A map derived from an independent F-2 progeny from one of the crosses was also used. With a total of 521 genotyped individuals, accuracy in gene order is expected. Five sources of markers were used: (i) 109 loci corresponding to 69 genes of known function, (ii) 39 loci controlling protein position shifts revealed by two-dimensional electrophoresis, (iii) 8 isozyme loci, (iv) 17 loci corresponding to 14 sequenced cDNAs for which no homology was found in gene banks, and (v) 102 loci corresponding to 81 anonymous probes. As many loci were common to all maps, we tested heterogeneity between recombination fractions. The comparison of recombination fractions revealed: (i) a good correspondence between the maps derived from the same cross, (ii) few significant differences in interval distances, and (iii) global differences, which can reach 20% of the total map length. A composite map of 275 loci covering 1765 cM has been constructed.

引用

页码：418 / 432

页数：15

共 86 条

[1] COMPARATIVE LINKAGE MAPS OF THE RICE AND MAIZE GENOMES [J].