Construction and characterization of Escherichia coli disruptants defective in the yaeM gene

被引：32

作者：

Kuzuyama, T ^{[1
]}

Takahashi, S ^{[1
]}

Seto, H ^{[1
]}

机构：

[1] Univ Tokyo, Inst Mol & Cellular Biosci, Bunkyo Ku, Tokyo 1130032, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1999年 / 63卷 / 04期

关键词：

biosynthesis; terpenoid; nonmevalonate pathway; 2-C-methyl-D-erythritol; 4-phosphate; Escherichia coli;

D O I：

10.1271/bbb.63.776

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Escherichia coli disruptants defective in the yaeM gene, which is located at 4.2 min on the chromosome map, were constructed and characterized. The disruptants showed auxotrophy for 2-C-methylerythritol, a free alcohol of 2-C-methyl-D-erythritol 4-phosphate that is a biosynthetic precursor in the nonmevalonate pathway. This result clearly shows that the yaeM gene is indeed involved in this pathway in E. coli.

引用

页码：776 / 778

页数：3

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