Two-dimensional protein analysis by isoelectric focusing in a multi-cell plate system

被引：1

作者：

Ferreira, BD ^{[1
]}

Branco, AT ^{[1
]}

de Queiroz, KS ^{[1
]}

de Souza, GA ^{[1
]}

机构：

[1] Univ Estadual Norte Fluminense, Lab Biotecnol, Ctr Biociencias & Biotecnol, BR-28015620 Campos dos Goytacazes, RJ, Brazil

来源：

PROTEIN AND PEPTIDE LETTERS | 2002年 / 9卷 / 01期

关键词：

isoelectric protein focusing; two dimensional; protein; multi-cell plate; IEF system;

D O I：

10.2174/0929866023409057

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A practical and low cost system for isoelectric protein focusing (IEF) was developed. The system uses a multi-cell glass plate compatible with a common vertical one-dimensional electrophoresis chamber, dispensing specific IEF apparatus. After focusing, the IEF gels are easily recovered. The resulting two-dimensional (2-D) gets have provided efficient protein separation for different concentrations and extracts.

引用

页码：39 / 43

页数：5

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