Binding proteins selected from combinatorial libraries of an alpha-helical bacterial receptor domain

Small protein domains, capable of specific binding to different target proteins have been selected using combinatorial approaches. These binding proteins, Galled affibodies, were designed by randomization of 13 solvent-accessible surface residues of a stable alpha-helical bacterial receptor domain Z, derived from staphylococcal protein A. Repertoires of mutant Z domain genes were assembled and inserted into a phagemid vector adapted for monovalent phage display. Two libraries, each comprising approximately 4x10(7) transformants, were constructed using either an NN(G/T) or an alternative (C/A/G)NN degeneracy Biopanning against: the target proteins Tag DNA polymerase, human insulin, and a human apolipoprotein A-1 variant, showed that in all cases significant enrichments were obtained by file selection procedures. Selected clones were subsequently expressed in Escherichia coli and analyzed by SDS-PAGE, circular dichroism spectroscopy, and binding studies to their respective targets by biospecific interaction analysis. The affibodies have a secondary structure similar to the native Z domain and have micromolar dissociation constants (K-D) for their respective targets.