A semi-defined medium without serum for small ruminant mycoplasmas

被引：7

作者：

Ramirez, Ana S.

Luis Fleitas, Juan

Rosales, Ruben S.

Poveda, Carlos

de la Fe, Christian

Andrada, Marisa ^{[1
]}

Castro, Ayoze ^{[1
]}

Poveda, Jose B.

机构：

[1] Univ Las Palmas Gran Canaria, Fac Vet, Dept Histol & Patol, Las Palmas Gran Canaria 35416, Spain

来源：

VETERINARY JOURNAL | 2008年 / 178卷 / 01期

关键词：

Mollicutes; Mycoplasma species; Culture media; Animal sera;

D O I：

10.1016/j.tvjl.2007.07.023

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

The composition of the medium used to cultivate Mycoplasma species is very important. Serum is one of the most important additives as it contains lipids (cholesterol) and serum proteins, which are essential for the growth of the organisms. This work reports the development of a semi-defined medium, called MWS (Medium Without Serum) produced without animal serum and bovine serum albumin. MWS seems to be suitable for cultivating several species of caprine mycoplasma, especially M. mycoides subsp. mycoides (LC) and M. mycoides subsp. capri. (C) 2007 Elsevier Ltd. All rights reserved.

引用

页码：149 / 152

页数：4

共 29 条

[1] SENSITIVITY AND SPECIFICITY OF MYCOPLASMA-GALLISEPTICUM AGGLUTINATION ANTIGENS PREPARED FROM MEDIUM WITH ARTIFICIAL LIPOSOMES SUBSTITUTING FOR SERUM [J].