Mucociliary transport in porcine trachea: differential effects of inhibiting chloride and bicarbonate secretion

Cooper JL, Quinton PM, Ballard ST. Mucociliary transport in porcine trachea: differential effects of inhibiting chloride and bicarbonate secretion. Am J Physiol Lung Cell Mol Physiol 304: L184-L190, 2013. First published November 30, 2012; doi: 10.1152/ajplung.00143.2012.-This study was designed to assess the relative importance of Cl- and HCO3- secretion to mucociliary transport rate (MCT) in ex vivo porcine tracheas. MCT was measured in one group of tissues that was exposed to adventitial HCO3--free solution while a parallel group was exposed to adventitial HCO3--replete solution. After measurement of baseline MCT rates, acetylcholine (ACh) was added to stimulate submucosal gland mucous liquid secretion, and MCT rates were again measured. Before ACh addition, the mean MCT was higher in the HCO3--free group (4.2 +/- 0.9 mm/min) than in the HCO3--replete group (2.3 +/- 0.3 mm/min), but this difference was not statistically significant. ACh addition significantly increased MCT in both groups, but ACh-stimulated MCT was significantly lower in the HCO3--free group (11.0 +/- 1.5 mm/min) than in the HCO3--replete group (17.0 +/- 2.0 mm/min). A second series of experiments examined the effect on MCT of blocking Cl- secretion with 100 mu M bumetanide. Before adding ACh, MCT in the bumetanide-treated group (1.0 +/- 0.2 mm/min) was significantly lower than in the control group (3.8 +/- 1.1 mm/min). ACh addition significantly increased MCT in both groups, but there was no significant difference between the bumetanide-treated group (21.4 +/- 1.7 mm/min) and control group (19.5 +/- 3.4 mm/min). These results indicate that ACh-stimulated MCT has greater dependence on HCO3- secretion, whereas the basal MCT rate has greater dependence on Cl- secretion.