Vascular endothelial growth factor A (VEGF-A) mRNA expression levels decrease after menopause in normal breast tissue but not in breast cancer lesions

被引:23
作者
Greb, RR [1 ]
Maier, I [1 ]
Wallwiener, D [1 ]
Kiesel, L [1 ]
机构
[1] Univ Tubingen Hosp, Dept Obstet & Gynecol, D-72076 Tubingen, Germany
关键词
breast cancer; angiogenesis; VEGF; steroid hormones; menopause;
D O I
10.1038/sj.bjc.6690681
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
We hypothesized that the regulation of microvascular functions and angiogenesis in breast tissue, a well known target of ovarian steroid action, is dependent on the hormonal exposure of the breast. Relative expression levels of VEGF-A (vascular endothelial growth factor A), a putative key regulator of angiogenesis in breast cancer, were analysed in the tumour and the adjacent non-neoplastic breast tissue of 19 breast cancer patients by quantitative reverse transcriptase polymerase chain reaction. In non-neoplastic breast specimens the expression levels of all detected VEGF-A-isoforms (189, 165, 121) were significantly higher in premenopausal compared to post-menopausal women (P = 0.02) and were inversely correlated with the patient's age (P = 0.006). In contrast, in cancerous tissues menopausal status had no influence on VEGF-A-expression levels. Benign and malignant tissues exhibited a similar expression pattern of VEGF-A-isoforms relative to each other. Thus, the regulation of the vasculature in normal breast tissue, as opposed to breast cancer tissue, appears to be hormonally dependent. Endogenous and therapeutically used hormonal steroids might, therefore, cause clinically relevant changes of the angiogenic phenotype of the human breast. (C) 1999 Cancer Research Campaign.
引用
收藏
页码:225 / 231
页数:7
相关论文
共 35 条
[1]   Vascular endothelial growth factor and platelet-derived growth factor are potential angiogenic and metastatic factors in human breast cancer [J].
Anan, K ;
Morisaki, T ;
Katano, M ;
Ikubo, A ;
Kitsuki, H ;
Uchiyama, A ;
Kuroki, S ;
Tanaka, M ;
Torisu, M .
SURGERY, 1996, 119 (03) :333-339
[2]  
Beatson GT., 1896, LANCET, V148, P104, DOI DOI 10.1016/S0140-6736(01)72307-0
[3]  
Benoy I, 1998, EUR J CANCER, V34, P1298
[4]   Protein expression profiles in human breast ductal carcinoma and histologically normal tissue [J].
Bini, L ;
Magi, B ;
Marzocchi, B ;
Arcuri, F ;
Tripodi, S ;
Cintorino, M ;
Sanchez, JC ;
Frutiger, S ;
Hughes, G ;
Pallini, V ;
Hochstrasser, DF ;
Tosi, P .
ELECTROPHORESIS, 1997, 18 (15) :2832-2841
[5]   EXPRESSION OF VASCULAR-PERMEABILITY FACTOR (VASCULAR ENDOTHELIAL GROWTH-FACTOR) AND ITS RECEPTORS IN BREAST-CANCER [J].
BROWN, LF ;
BERSE, B ;
JACKMAN, RW ;
TOGNAZZI, K ;
GUIDI, AJ ;
DVORAK, HF ;
SENGER, DR ;
CONNOLLY, JL ;
SCHNITT, SJ .
HUMAN PATHOLOGY, 1995, 26 (01) :86-91
[6]   Breast lesions: Correlation of contrast medium enhancement patterns on MR images with histopathologic findings and tumor angiogenesis [J].
Buadu, LD ;
Murakami, J ;
Murayama, S ;
Hashiguchi, N ;
Sakai, S ;
Masuda, K ;
Toyoshima, S ;
Kuroki, S ;
Ohno, S .
RADIOLOGY, 1996, 200 (03) :639-649
[7]  
Chung HC, 1998, INT J MOL MED, V2, P465
[8]   REGULATION OF TUMOR HOST INTERACTIONS IN BREAST-CANCER [J].
DICKSON, RB .
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY, 1992, 41 (3-8) :389-400
[9]   The biology of vascular endothelial growth factor [J].
Ferrara, N ;
DavisSmyth, T .
ENDOCRINE REVIEWS, 1997, 18 (01) :4-25
[10]   Prognostic significance of vascular endothelial growth factor protein in node-negative breast carcinoma [J].
Gasparini, G ;
Toi, M ;
Gion, M ;
Verderio, P ;
Dittadi, R ;
Hanatani, M ;
Matsubara, I ;
Vinante, O ;
Bonoldi, E ;
Boracchi, P ;
Gatti, C ;
Suzuki, H ;
Tominaga, T .
JOURNAL OF THE NATIONAL CANCER INSTITUTE, 1997, 89 (02) :139-147