Isolation and genomic characterization of Chaoyang virus strain ROK144 from Aedes vexans nipponii from the Republic of Korea

被引:40
作者
Lee, John S. [5 ]
Grubaugh, Nathan D. [1 ]
Kondig, John P. [2 ]
Turell, Michael J. [1 ]
Kim, Heung-Chul [3 ]
Klein, Terry A. [4 ]
O'Guinn, Monica L. [5 ]
机构
[1] USA, Div Virol, Med Res Inst Infect Dis, Ft Detrick, MD 21702 USA
[2] USA, Diagnost Syst Div, Med Res Inst Infect Dis, Ft Detrick, MD 21702 USA
[3] 168th Multifunct Med Battal, Med Detachment 5, Seoul, South Korea
[4] 65th Med Brigade USAMEDDAC Korea, Force Hlth Protect & Prevent Med, Seoul, South Korea
[5] Mil Infect Dis Res Program, Ft Detrick, MD 21702 USA
关键词
Arthropod-specific flavivirus; Mosquito flavivirus; Vector surveillance; Arbovirus; NATURAL MOSQUITO POPULATION; AMAZON BASIN REGION; FUSING AGENT VIRUS; INSECT FLAVIVIRUS; CULEX;
D O I
10.1016/j.virol.2012.10.020
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
During June 2003, mosquito surveillance was conducted at a US Army installation and a US Military training site 2 km south of the demilitarized zone, Republic of Korea. Mosquitoes were collected using Mosquito Magnets (TM), sorted to species, and assayed for the presence of arboviruses. From the 3,149 mosquitoes that were sorted into 126 pools, one Aedes vexans nipponii pool (out of 73 pools) tested positive for flavivirus RNA by reverse transcription-PCR. After isolation from C6/36 cell culture supernatant, the viral genome was sequenced and found to be 98.9% related to Chaoyang virus, a potential arthropod-specific flavivirus. This report details the first identification of Chaoyang virus in the Republic of Korea and highlights its relationship to other flaviviruses. (C) 2012 Elsevier Inc. All rights reserved.
引用
收藏
页码:220 / 224
页数:5
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