Development of a high-performance liquid chromatographic method for the determination of a new potent radioiodinated melanoma imaging and therapeutic agent

被引：11

作者：

Denoyer, Delphine ^{[1
]}

Labarre, Pierre ^{[1
]}

Papon, Janine ^{[1
]}

Miot-Noirault, Elisabeth ^{[1
]}

Galmier, Marie-Josephe ^{[1
]}

Madelmont, Jean-Claude ^{[1
]}

Chezal, Jean-Michel ^{[1
]}

Moins, Nicole ^{[1
]}

机构：

[1] Univ Auvergne, Ctr Jean Perrin, INSERM, EA 4231, F-63005 Clermont Ferrand, France

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2008年 / 875卷 / 02期

关键词：

Radiopharmaceutical targeting melanoma; N-(2-diethylaminoethyl)-6-iodoquinoxaline-2-carboxamide; ICF; 01012; HPLC analysis;

D O I：

10.1016/j.jchromb.2008.09.021

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

N-(2-diethylaminoethyl)-6-iodoquinoxaline-2-carbamide(ICF 01012) is a new melanoma imaging agent showing promising properties for application in internal radionuclide therapy. We developed an analytical protocol for detection of ICF 01012 in biological samples using HPLC. The proposed method was first validated using standard of ICF 01012 and four potent metabolites of this compound and then applied to follow the metabolic fate of [(125)I]ICF 01012 after injection in melanoma-bearing mice. The results demonstrate that this method exhibits a good linearity (r(2) = 0.9947), specificity and acceptable accuracy. This simple method appears convenient and sufficient for pharmacokinetic studies on [(125)I]ICF 01012. (c) 2008 Elsevier B.V. All rights reserved.

引用

页码：411 / 418

页数：8

共 20 条

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[2]

Clinical study of [123I] N-(2-diethylaminoethyl)-4-iodobenzamide in the diagnosis of primary and metastatic ocular melanoma [J].