The uvrA gene is involved in oxidative and acid stress responses in Lactobacillus helveticus CNBL1156

被引：42

作者：

Cappa, F

Cattivelli, D

Cocconcelli, PS

机构：

[1] Istituto di Microbiologia, Università Cattolica del Sacro Cuore, 29100 Piacenza

[2] Centro Ricerche Biotecnologiche, Università Cattolica del Sacro Cuore, 26100 Cremona

来源：

RESEARCH IN MICROBIOLOGY | 2005年 / 156卷 / 10期

关键词：

Acid response; Lactobacillus helveticus; Oxidative stress; UvrA;

D O I：

10.1016/j.resmic.2005.06.003

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The uvrA gene of Lactobacillus helveticus CNBL1156 coding for subunit A of the excinuclease ABC complex involved in the nucleotide excision repair mechanism was identified. Analysis of the uvrA locus revealed the presence of three open reading frames, merR, sat and uvrA, which coded respectively for a MerR-like regulatory protein, a putative protein with homology to streptothricin acetyl transferase and for a UvrA protein. RNA analysis by northern blotting and RT-PCR showed that sat and uvrA were transcriptionally coupled. UvrA from L. helveticus contained the conserved domains of bacterial excinuclease A, as well as the two ATP binding sites and the zinc binding domains. The transcriptional activity of uvrA indicated that this gene was activated by exposure to UV radiation and oxidative stress. In addition, we observed that the expression of uvrA was inducible by pH; moreover, the role of UvrA in protection against stress was confirmed by acid adaptation experiments. Pretreatment of cells at pH 5 conferred resistance to H2O2, suggesting a specific adaptive response to pH-induced DNA damage. The results from this study indicate that UvrA contributes to acid and oxidative tolerance in L. helveticus, and suggest that it plays a role in survival at low pH under normal conditions. © 2005 Elsevier SAS. All rights reserved.

引用

页码：1039 / 1047

页数：9

共 40 条

[1] Ahmed M., Lyass L., Markham P.N., Taylor S.S., Vazquez-Laslop N., Neyfakh A.A., Two highly similar multidrug transporters of Bacillus subtilis whose expression is differentially regulated, J. Bacteriol., 177, pp. 3904-3910, (1995)
[2] Altschul S.F., Madden T.L., Schaffer A.A., Zhang J., Miller W., Lipman D., Gapped BLAST and PSI-BLAST: A new generation of protein database search programs, Nucleic Acids Res., 25, pp. 3389-3402, (1997)
[3] Bolotin A., Wincker P., Mauger S., Jaillon Malarme O.K., Weissenbach J., Ehrlich S.D., Sorokin A., The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403, Genome Res., 11, pp. 731-753, (2001)
[4] Smith B.T., Grossman A.D., Walker G.C., Localization of UvrA and effect of DNA damage on the chromosome of Bacillus subtilis, J. Bacteriol., 184, pp. 488-493, (2002)
[5] Brown N.L., Stoyanov J.V., Kidd S.P., Hobman J.L., The MerR family of transcriptional regulators, FEMS Microbiol. Rev., 27, pp. 145-163, (2003)
[6] Cocconcelli P.S., Parisi M.G., Senini L., Bottazzi V., Use of RAPD and 16S rDNA sequencing for the study of Lactobacillus population dynamics in natural whey culture, Lett. Appl. Microbiol., 25, pp. 8-12, (1997)
[7] Cotter P.D., Hill C., Surviving the acid test: Responses of Gram-positive bacteria to low pH, Microbiol. Mol. Biol. Rev., 67, pp. 429-453, (2003)
[8] Dower W.J., Miller J.F., Rasdale W., High efficiency transformation of E. coli by high voltage electroporation, Nucleic Acids Res., 16, pp. 6127-6145, (1988)
[9] Foster J.W., Hall H.K., Inducible pH homeostasis and the acid tolerance response of Salmonella typhimurium, J. Bacteriol., 172, pp. 771-778, (1991)
[10] Foster J.W., When protons attack: Microbial strategies of acid adaptation, Curr. Opin. Microbiol., 2, pp. 170-174, (1999)

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