Quantitative structure/retention relationships in affinity chromatography

被引：28

作者：

Baczek, T ^{[1
]}

Kaliszan, R ^{[1
]}

机构：

[1] Med Univ Gdansk, Dept Biopharmaceut & Pharmacodynam, PL-80416 Gdansk, Poland

来源：

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 2001年 / 49卷 / 1-3期

关键词：

high-performance affinity chromatography; intermolecular interactions; quantitative structure/enantiospecific; retention relationships; quantitative structure/retention relationships; chirality; enantiospecific stationary phascs; immobilized proteins; structural descriptors;

D O I：

10.1016/S0165-022X(01)00190-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Affinity chromatography (AC) followed by quantitative structure/retention relationships (QSRR) analysis provides information on both the analytes and the macromolecules forming the stationary phases. QSRR equations derived for test series of analytes (often drugs) are interpreted in terms of structural requirements of the specific binding sites on macromolecules. Chromatographically demonstrated differences in analyte/macromolecule interactions may be relevant to molecular pharmacology and rational drug design. Multiple regression analysis of appropriately designed sets of affinity-chromatographic data may help increase the speed and efficiency of search Lis for new drugs and reduce the need for in vivo screening. Specific high-performance affinity-chromatographic separations can be optimized by rational selection of chiral columns, the characteristics of which are provided by QSRR. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：83 / 98

页数：16

共 44 条

[1] CIRCULAR-DICHROISM AND GEL-FILTRATION STUDY OF BINDING OF PROCHIRAL AND CHIRAL 1,4-BENZODIAZEPIN-2-ONES TO HUMAN-SERUM ALBUMIN [J].