LNA (Locked Nucleic Acid)

被引：41

作者：

Wengel, J

Koshkin, A

Singh, SK

Nielsen, P

Meldgaard, M

Rajwanshi, VK

Kumar, R

Skouv, J

Nielsen, CB

Jacobsen, JP

Jacobsen, N

Olsen, CE

机构：

[1] Univ Copenhagen, Ctr Synthet Bioorgan Chem, Dept Chem, DK-2100 Copenhagen, Denmark

[2] Exiqon AS, DK-2950 Vedbaek, Denmark

[3] Odense Univ, Dept Chem, DK-5230 Odense, Denmark

[4] Danish Canc Soc, Inst Canc Biol, DK-2100 Copenhagen, Denmark

[5] Royal Vet & Agr Univ, Dept Chem, DK-1871 Frederiksberg C, Denmark

来源：

NUCLEOSIDES & NUCLEOTIDES | 1999年 / 18卷 / 6-7期

关键词：

D O I：

10.1080/07328319908044718

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

LNA (Locked Nucleic Acid) forms duplexes with complementary DNA, RNA or LNA with unprecedented thermal affinities. CD spectra show that duplexes involving fully modified LNA (especially LNA:RNA) structurally resemble an A-form RNA:RNA duplex. NMR examination of an LNA:DNA duplex confirm the 3'-endo conformation of an LNA monomer. Recognition of double-stranded DNA is demonstrated suggesting strand invasion by LNA. Lipofectin-mediated efficient delivery of LNA into living human breast cancer cells has been accomplished.

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页码：1365 / 1370

页数：6

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