Acylphosphate formation by the Menkes copper ATPase

被引：25

作者：

Solioz, M ^{[1
]}

Camakaris, J ^{[1
]}

机构：

[1] UNIV MELBOURNE,DEPT GENET,PARKVILLE,VIC 3052,AUSTRALIA

来源：

FEBS LETTERS | 1997年 / 412卷 / 01期

基金：

澳大利亚研究理事会;

关键词：

Menkes disease; P-type ATPase; copper; phosphorylation; copper resistance; (hamster);

D O I：

10.1016/S0014-5793(97)00770-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Menkes ATPase is the product of the MNK gene, defective in some inherited human disorders of copper metabolism. We here show the formation of an acylphosphate intermediate by the murine MNK homologue in membranes from normal and copper resistant Chinese hamster ovary cells. In the latter, fivefold higher levels of acylphosphate were formed. Challenging these cells with copper, which induces relocation of the MNK ATPase from the trans-Golgi network to the plasma membrane, did not influence acylphosphate formation. The kinetics of phosphorylation, metal dependence, and sensitivity to inhibitors were investigated. The results show that the MNK ATPase is an active P-type ATPase and provide a direct functional test for this enzyme. (C) 1997 Federation of European Biochemical Societies.

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页码：165 / 168

页数：4

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