Clinical Utility of Insulin-Like Growth Factor 1 and 2; Determination by High Resolution Mass Spectrometry

被引：54

作者：

Bystrom, Cory ^{[1
]}

Sheng, Shijun ^{[2
]}

Zhang, Ke ^{[3
]}

Caulfield, Michael ^{[3
]}

Clarke, Nigel J. ^{[3
]}

Reitz, Richard ^{[3
]}

机构：

[1] Cleveland Heart Lab, Cleveland, OH USA

[2] Thermo Sci, San Jose, CA USA

[3] Quest Diagnost Nichols Inst, San Juan Capistrano, CA USA

来源：

PLOS ONE | 2012年 / 7卷 / 09期

关键词：

IGF-I ASSAYS; REFERENCE VALUES; PROTEINS; PLASMA; SERUM; RADIOIMMUNOASSAY; QUANTIFICATION; RADIORECEPTOR; THYROGLOBULIN; HYPOGLYCEMIA;

D O I：

10.1371/journal.pone.0043457

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

Measurement of insulin-like growth factor-1 (IGF-I) has utility for the diagnosis and management of growth disorders, but inter-assay comparison of results has been complicated by a multitude of reference standards, antibodies, detection methods, and pre-analytical preparation strategies. We developed a quantitative LC-MS method for intact IGF-I, which has advantages in throughput and complexity when compared to mass spectrometric approaches that rely on stable isotope dilution analysis of tryptic peptides. Since the method makes use of full-scan data, the assay was easily extended to provide quantitative measurement of IGF-II using the same assay protocol. The validated LC-MS assay for IGF-I and IGF-II provides accurate results across the pediatric and adult reference range and is suitable for clinical use.

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