Biodegradation of cyanide compounds by a Pseudomonas species (S1)

被引:22
作者
Dhillon, JK [1 ]
Shivaraman, N [1 ]
机构
[1] Natl Environm Engn Res Inst, Environm Biotechnol Div, Nagpur 440020, Maharashtra, India
关键词
Pseudomonas sp.(S1); biodegradation; biotransformation; nitriles; cyanide;
D O I
10.1139/cjm-45-3-201
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A Pseudomonas sp. (S1), isolated from soil by an enrichment technique was tested for its potential to degrade different cyanide compounds. Further, biodegradation/biotransformation of binary mixtures of the cyanide compounds by the culture was also studied. The results indicated that the culture could grow on the following nitriles by using them as carbon and nitrogen sources: acetonitrile, butyronitrile, acrylonitrile, adiponitrile, benzonitrile, glutaronitrile, phenylacetonitrile, and succinonitrile. Studies on the biodegradation of these cyanide compounds in binary mixtures showed that the presence of acrylonitrile or KCN delayed the degradation of acetonitrile in a mixture, while none of the other cyanide compounds affected the degradation of one another. The transformation products of the nitriles were their corresponding acids, and similarly, KCN was also directly transformed to formic acid. Studies on the transformation of these cyanide compounds showed that the rate of transformation of nitriles to their corresponding carboxylic acids was acrylonitrile > acetonitrile > adiponitrile > benzonitrile > KCN. This culture has the unique characteristic of transforming representatives of saturated aliphatic, aliphatic olefinic, aromatic, and aralkyl nitriles, as well as alkali cyanide, to their corresponding carboxylic acids.
引用
收藏
页码:201 / 208
页数:8
相关论文
共 30 条
[1]  
*APHA AWWA WPCF, 1992, STAND METH EX WAT WA
[2]   SUBSTRATE INTERACTIONS DURING AEROBIC BIODEGRADATION OF BENZENE [J].
ARVIN, E ;
JENSEN, BK ;
GUNDERSEN, AT .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1989, 55 (12) :3221-3225
[3]   MICROBIAL-DEGRADATION OF NITRILE COMPOUNDS .7. A NEW ENZYMATIC METHOD OF ACRYLAMIDE PRODUCTION [J].
ASANO, Y ;
YASUDA, T ;
TANI, Y ;
YAMADA, H .
AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1982, 46 (05) :1183-1189
[4]   MICROBIAL DEGREDATION OF NITRILE COMPOUNDS .5. ALIPHATIC NITRILE HYDRATASE FROM ARTHROBACTER SP J-1 PURIFICATION AND CHARACTERIZATION [J].
ASANO, Y ;
FUJISHIRO, K ;
TANI, Y ;
YAMADA, H .
AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1982, 46 (05) :1165-1174
[5]   BACTERIAL CYANIDE DETOXIFICATION [J].
ATKINSON, A .
BIOTECHNOLOGY AND BIOENGINEERING, 1975, 17 (03) :457-460
[6]   MICROBIAL-METABOLISM OF NITRILES AND CYANIDES [J].
BABU, GRV ;
CHETTY, CS ;
WOLFRAM, JH ;
CHAPATWALA, KD .
JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART A-ENVIRONMENTAL SCIENCE AND ENGINEERING & TOXIC AND HAZARDOUS SUBSTANCE CONTROL, 1994, 29 (09) :1957-1971
[7]  
BABU GRV, 1995, APPL MICROBIOL BIOT, V43, P739
[8]   PURIFICATION AND CHARACTERIZATION OF BENZONITRILASES FROM ARTHROBACTER SP STRAIN J-1 [J].
BANDYOPADHYAY, AK ;
NAGASAWA, T ;
ASANO, Y ;
FUJISHIRO, K ;
TANI, Y ;
YAMADA, H .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1986, 51 (02) :302-306
[9]  
BUI K, 1984, J GEN MICROBIOL, V130, P89
[10]  
DHILLON JK, 1996, P 3 INT C ENV PLANN, P223