Mesendoderm extension and mantle closure in Xenopus laevis gastrulation:: Combined roles for integrin α5β1, fibronectin, and tissue geometry

被引:117
作者
Davidson, LA
Hoffstrom, BG
Keller, R
DeSimone, DW [1 ]
机构
[1] Univ Virginia Hlth Syst, Sch Med, Dept Cell Biol, Charlottesville, VA 22908 USA
[2] Univ Virginia, Dept Biol, Charlottesville, VA 22903 USA
关键词
mesendoderm; integrin; fibronectin; cell migration; radial intercalation;
D O I
10.1006/dbio.2002.0537
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
We describe mesendoderm morphogenesis during gastrulation in the frog Xenopus laevis and investigate the mechanics of these movements with tissue explants. When a dorsal marginal zone explant is plated onto fibronectin, the mesendoderm moves away from the dorsal axial tissues as an intact sheet. Mesendodermal cells within these explants display monopolar protrusive activity and radially intercalate during explant extension. Live time-lapse confocal sequences of actin dynamics at the margin of these extending explants prompt us to propose that integrin-mediated traction drives these movements. We demonstrate that integrin alpha(5)beta(1) recognition of the synergy site located within the type III9 repeat of fibronectin is required for mesendoderm extension. Normal mesendoderm morphogenesis occurs with a unique "cup-shaped" geometry of the extending mesendodermal mantle and coincides with a higher rate of tissue extension than that seen in the simpler dorsal marginal zone explant. These higher rates can be reconstituted with "in-the-round" configurations of several explants. We propose several mechanically based hypotheses to explain both the initial fibronectin-dependent extension of the mesendoderm and additional requirement of tissue geometry during the high-velocity closure of the mesendodermal mantle. (C) 2002 Elsevier Science (USA).
引用
收藏
页码:109 / 129
页数:21
相关论文
共 58 条
[1]  
BAUER DV, 1994, DEVELOPMENT, V120, P1179
[2]  
BOUCAUT J-C, 1990, International Journal of Developmental Biology, V34, P139
[3]  
BOUCAUT JC, 1983, CELL TISSUE RES, V234, P135
[4]   BIOLOGICALLY-ACTIVE SYNTHETIC PEPTIDES AS PROBES OF EMBRYONIC-DEVELOPMENT - A COMPETITIVE PEPTIDE INHIBITOR OF FIBRONECTIN FUNCTION INHIBITS GASTRULATION IN AMPHIBIAN EMBRYOS AND NEURAL CREST CELL-MIGRATION IN AVIAN EMBRYOS [J].
BOUCAUT, JC ;
DARRIBERE, T ;
POOLE, TJ ;
AOYAMA, H ;
YAMADA, KM ;
THIERY, JP .
JOURNAL OF CELL BIOLOGY, 1984, 99 (05) :1822-1830
[5]   PREVENTION OF GASTRULATION BUT NOT NEURULATION BY ANTIBODIES TO FIBRONECTIN IN AMPHIBIAN EMBRYOS [J].
BOUCAUT, JC ;
DARRIBERE, T ;
BOULEKBACHE, H ;
THIERY, JP .
NATURE, 1984, 307 (5949) :364-367
[6]   Extracellular matrix rigidity causes strengthening of integrin-cytoskeleton linkages [J].
Choquet, D ;
Felsenfeld, DP ;
Sheetz, MP .
CELL, 1997, 88 (01) :39-48
[7]   REQUIREMENT FOR THE SYNERGY SITE FOR CELL-ADHESION TO FIBRONECTIN DEPENDS ON THE ACTIVATION STATE OF INTEGRIN ALPHA-5-BETA-1 [J].
DANEN, EHJ ;
AOTA, SI ;
VANKRAATS, AA ;
YAMADA, KM ;
RUITER, DJ ;
VANMUIJEN, GNP .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (37) :21612-21618
[8]   THE 140-KDA FIBRONECTIN RECEPTOR COMPLEX IS REQUIRED FOR MESODERMAL CELL-ADHESION DURING GASTRULATION IN THE AMPHIBIAN PLEURODELES-WALTLII [J].
DARRIBERE, T ;
YAMADA, KM ;
JOHNSON, KE ;
BOUCAUT, JC .
DEVELOPMENTAL BIOLOGY, 1988, 126 (01) :182-194
[9]   Integrins:: Regulators of embryogenesis [J].
Darribère, T ;
Skalski, M ;
Cousin, H ;
Gaultier, A ;
Montmory, C ;
Alfandari, D .
BIOLOGY OF THE CELL, 2000, 92 (01) :5-25
[10]   INVIVO ANALYSES OF INTEGRIN BETA-1 SUBUNIT FUNCTION IN FIBRONECTIN MATRIX ASSEMBLY [J].
DARRIBERE, T ;
GUIDA, K ;
LARJAVA, H ;
JOHNSON, KE ;
YAMADA, KM ;
THIERY, JP ;
BOUCAUT, JC .
JOURNAL OF CELL BIOLOGY, 1990, 110 (05) :1813-1823