Regulation of Sp100A Subnuclear Localization and Transcriptional Function by EBNA-LP and Interferon

Epstein-Barr virus (EBV) efficiently immortalizes human B cells and is associated with several human malignancies. The EBV transcriptional activating protein EBNA2 and the EBNA2 coactivator EBNA-leader protein (EBNA-LP) are important for B cell immortalization. Recent observations from our laboratory indicate that EBNA-LP coactivation function is mediated through interactions with the interferon-inducible gene (ISG) Sp100, resulting in displacement from its normal location in promyelocytic leukemia nuclear bodies (PML NBs) into the nucleoplasm. The EBNA-LP- and interferon-mediated mechanisms that regulate Sp100 subnuclear localization and transcriptional function remain undefined. To clarify these issues, we generated a panel of Sp100 mutant proteins to ascertain whether EBNA- LP induces Sp100 displacement from PML NBs by interfering with Sp100 dimerization or through other domains. In addition, we tested EBNA- LP function in interferon-treated cells. Our results indicate that Sp100 dimerization, PML NB localization, and EBNA- LP interaction domains overlap significantly. We also show that IFN-beta does not inhibit EBNA- LP coactivation function. The results suggest that EBNA- LP might play a role in EBV-evasion of IFN-mediated antiviral responses.