Screening of Arabidopsis thaliana stems for variation in cell wall polysaccharides

被引：28

作者：

Gardner, SL

Burrell, MM

Fry, SC

机构：

[1] Univ Edinburgh, Inst Cell & Mol Biol, Edinburgh Cell Wall Grp, Edinburgh EH9 3JH, Midlothian, Scotland

[2] Adv Technol Cambridge Ltd, Cambridge CB4 0WA, England

来源：

PHYTOCHEMISTRY | 2002年 / 60卷 / 03期

基金：

英国生物技术与生命科学研究理事会;

关键词：

Arabidopsis thaliana; Cruciferae; cell walls; polysaccharides; xyloglucan; xylan; cellulose; pectins; Driselase; screening; chromatography;

D O I：

10.1016/S0031-9422(02)00046-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A high-throughput method is described by which Arabidopsis thaliana stems can be screened for variation in cell wall composition after hydrolysis with Driselase or trifluoroacetic acid (TFA). Driselase, a mixture of fungal enzymes, hydrolyses cellulose (to glucose) and all the major matrix polysaccharides (to monosaccharides and/or characteristic disaccharides); TFA hydrolyses the matrix polysaccharides, but not cellulose, to monosaccharides. Two different wild-type ecotypes, Columbia and Wassilewskija, showed only minor differences in wall carbohydrate composition. A small number of T-DNA-tagged populations that were screened contained individuals in which the proportion of cellulose, xyloglucan or xylan differed quantitatively from the wild-type. Differences from the wild-type were also observed in the susceptibility of the hemicelluloses to hydrolysis by Driselase, probably reflecting differences in wall architecture. (C) 2002 Elsevier Science Ltd. All rights reserved.

引用

页码：241 / 254

页数：14

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