Cloning and expression analysis of SKn-type dehydrin gene from bean in response to heavy metals

被引:45
作者
Zhang, YX
Li, JM
Yu, F
Cong, L
Wang, LY
Burkard, G
Chai, TY
机构
[1] Grad Univ Chinese Acad Sci, Dept Biol, Beijing 100049, Peoples R China
[2] China Univ Min & Technol, Sch Chem & Environm Engn, Dept Biol Engn, Beijing 100083, Peoples R China
[3] Univ Strasbourg, CNRS, Inst Biol Mol Plantes, F-67084 Strasbourg, France
关键词
heavy metal; dehydrin; Phaseolus vulgaris L;
D O I
10.1385/MB:32:3:205
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A heavy metal responsive gene PvSR3 (GenBank accession number U54703) encoding an acid dehydrin was isolated from a mercuric chloride-treated bean (Phaseolus vidgaris L.) leaf cDNA library by differential screening using cDNAs derived from treated and untreated plants. The PvSR3 cDNA is 981-bp long and has a 606-bp open-reading frame with a 202-residue-deduced amino acid sequence. The PvSR3 sequence contains two conserved repeats of the characteristic lysine-rich K segment (EKKGIMDKIKEKLPG) preceded by an 8-serine residue stretch, whereas the Y segment (DEYGNP) conserved motif is absent. The deduced protein has a calculated molecular weight of 23 kDa and an isoelectric point of 5.2. Sequence similarity and comparative analysis showed that PvSR3 shares 70 and 73% similarity with the dehydrin of poplar and pepper, respectively. Southern hybridizations indicated that PvSR3 was a low copy-number gene. Northern blot analysis revealed that PvSR3 mRNA was weakly detected in seedling leaves. However, the gene expression was strongly stimulated by heavy metals, such as mercury, cadmium, arsenic, and copper, whereas virus infection and salt had little effect oil it. In contrast, PvSR3 was not responsive to drought or abscisic acid (ABA), and was downregulated by UV radiation. Furthermore, PvSR3 was upregulated by the exogenous signaling molecules, including salicylic acid (SA) and hydrogen peroxide (H2O2). It is suggested that PvSR3 is extremely related to heavy metal stress, and might play an important role in metal detoxification and resistance to the damage caused by heavy metals.
引用
收藏
页码:205 / 217
页数:13
相关论文
共 40 条
[1]   Ion binding properties of the dehydrin ERD14 are dependent upon phosphorylation [J].
Alsheikh, MK ;
Heyen, BJ ;
Randall, SK .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (42) :40882-40889
[2]   Accumulation of dehydrin-like proteins in the mitochondria of cereals in response to cold, freezing, drought and ABA treatment [J].
Borovskii G.B. ;
Stupnikova I.V. ;
Antipina A.I. ;
Vladimirova S.V. ;
Voinikov V.K. .
BMC Plant Biology, 2 (1)
[3]   Dehydrins: genes, proteins, and associations with phenotypic traits [J].
Campbell, SA ;
Close, TJ .
NEW PHYTOLOGIST, 1997, 137 (01) :61-74
[4]   Dehydrin induction during drought and osmotic stress in Populus [J].
Caruso, A ;
Morabito, D ;
Delmotte, F ;
Kahlem, G ;
Carpin, S .
PLANT PHYSIOLOGY AND BIOCHEMISTRY, 2002, 40 (12) :1033-1042
[5]  
Chai TY, 2000, PROG NAT SCI-MATER, V10, P198
[6]   Cadmium resistance in transgenic tobacco plants enhanced by expressing bean heavy metal-responsive gene PvSR2 [J].
Chai, TY ;
Chen, Q ;
Zhang, YX ;
Dong, J ;
An, CC .
SCIENCE IN CHINA SERIES C-LIFE SCIENCES, 2003, 46 (06) :623-630
[7]  
Chai TY, 1998, PLANT SCI, V133, P47, DOI 10.1016/S0168-9452(98)00028-4
[8]   Drought tolerance established by enhanced expression of the CC-NBS-LRR gene, ADR1, requires salicylic acid, EDS1 and ABI1 [J].
Chini, A ;
Grant, JJ ;
Seki, M ;
Shinozaki, K ;
Loake, GJ .
PLANT JOURNAL, 2004, 38 (05) :810-822
[9]  
Chung E, 2003, MOL CELLS, V15, P327
[10]   Molecular mechanisms of plant metal tolerance and homeostasis [J].
Clemens, S .
PLANTA, 2001, 212 (04) :475-486