Rhinovirus-mediated changes in airway smooth muscle responsiveness:: induced autocrine role of interleukin-1β

被引:41
作者
Hakonarson, H
Carter, C
Maskeri, N
Hodinka, R
Grunstein, MM
机构
[1] Univ Penn, Sch Med, Childrens Hosp Philadelphia, Joseph Stokes Jr Res Inst,Div Pulm Med, Philadelphia, PA 19104 USA
[2] Univ Penn, Sch Med, Childrens Hosp Philadelphia, Joseph Stokes Jr Res Inst,Div Immunol & Infect Di, Philadelphia, PA 19104 USA
关键词
cholinergic contractility; beta-adrenoceptor relaxation; viral respiratory infection; cytokine signaling; airway reactivity; asthma;
D O I
10.1152/ajplung.1999.277.1.L13
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
An important interplay exists between specific viral respiratory pathogens, most commonly rhinovirus (RV), and altered airway responsiveness in the development and exacerbations of asthma. Given that RV infection reportedly induces the release of various cytokines in different cell types and that the reported effects of RV on airway smooth muscle (ASM) responsiveness are highly comparable to those obtained in ASM exposed to the proinflammatory cytokine interleukin (IL)-1 beta, this study examined whether RV (serotype 16)-mediated pertubations in ASM responsiveness are mechanistically coupled to altered induced expression and action of IL-1 beta in RV-exposed isolated rabbit and human ASM tissue and cultured cells. Relative to control tissues, ASM inoculated with RV exhibited significantly increased maximal isometric contractility to ACh (P < 0.01) and attenuated relaxation to isoproterenol (P < 0.005). In extended studies, we found that 1) the RV-induced changes in ASM responsiveness were ablated by pretreating the tissues with the IL-1 recombinant human receptor antagonist; 2) in contrast to their respective controls, RV-inoculated ASM tissue and cultured cells exhibited progressively induced expression of IL-1 beta mRNA and elaboration of IL-1 beta protein at 6 and 24 h after viral exposure; and 3) the latter effect of RV was inhibited in the presence of a monoclonal antibody to intercellular adhesion molecule-1, the endogenous receptor for most RV. Collectively, these observations provide new evidence demonstrating that "pro-asthmatic-like" pertubations in agonist responsiveness elicited in RV-exposed ASM are largely attributed to the induced autologous expression and autocrine action of IL-1 beta in the virus-infected ASM.
引用
收藏
页码:L13 / L21
页数:9
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