Evaluation of three immunochromatographic kits for rapid detection of influenza virus A and B

被引：8

作者：

Hara, Michimaru ^{[1
]}

Takao, Shinichi ^{[2
]}

Fukuda, Shinji ^{[2
]}

Shimazu, Yuki ^{[2
]}

Miyazaki, Kazuo ^{[2
]}

机构：

[1] Hiroshima Prefectural Inst Publ Hlth & Environm, Hara Pediat Clin, Hiroshima, Japan

[2] Hiroshima Prefectural Inst Publ Hlth & Environm, Dept Microbiol, Hiroshima, Japan

来源：

LABMEDICINE | 2008年 / 39卷 / 10期

关键词：

D O I：

10.1309/LM6ONMGY7K9ETMGF

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Background: Clinically, it is difficult to distinguish influenza infection from febrile infections caused by respiratory syncytial virus, adenovirus, rhinovirus, and human metapneumovirus without the use of rapid diagnostic tests. Methods: Nasopharyngeal aspirates (NPAs) were collected from 494 pediatric patients and were tested for the presence of influenza A and B virus antigen using 3 rapid diagnotic kits: ESPLINE Influenza A and B-N, Directigen EZ Flu A and B, and Binax NOW Influenza A and B. Results: Fifty-three specimens positive by viral culture were positive for influenza A by all 3 kits. Of the 270 influenza B virus cell-culture-positive specimens, the sensitivities of ESPLINE, EZ, and NOW were 86%, 76%, and 78%, respectively. Conclusion: Under well controlled conditions, influenza A virus sensitivities for all 3 kits can be 100% with specificities ranging from 98% to 100%.

引用

页码：603 / 606

页数：4

共 22 条

[1] Frequent detection of human rhinoviruses, paramyxoviruses, coronaviruses, and bocavirus during acute respiratory tract infections [J].

Arden, Katherine E. ;

McErlean, Peter ;

Nissen, Michael D. ;

Sloots, Theo P. ;

Mackay, Ian M. .

JOURNAL OF MEDICAL VIROLOGY, 2006, 78 (09) :1232-1240

[2] Comparison of two rapid influenza A/B test kits with reference methods showing high specificity and sensitivity for influenza A infection [J].

Booth, S ;

Baleriola, C ;

Rawlinson, WD .

JOURNAL OF MEDICAL VIROLOGY, 2006, 78 (05) :619-622

[3]

Cazacu AC, 2004, J CLIN MICROBIOL, V42, P3661, DOI [10.1128/JCM.42.8.3661-3664.2004, 10.1128/jcm.42.8.3661-3664.2004]

[4] Evaluation of the Directigen FluA+B test for rapid diagnosis of influenza virus type A and B infections [J].

Chan, KH ;

Maldeis, N ;

Pope, W ;

Yup, A ;

Ozinskas, A ;

Gill, J ;

Seto, WH ;

Shortridge, KF ;

Peiris, JSM .

JOURNAL OF CLINICAL MICROBIOLOGY, 2002, 40 (05) :1675-1680

[5] Comparison of four clinical specimen types for detection of influenza A and B viruses by optical immunoassay (FLU OIA test) and cell culture methods [J].

Covalciuc, KA ;

Webb, KH ;

Carlson, CA .

JOURNAL OF CLINICAL MICROBIOLOGY, 1999, 37 (12) :3971-3974

[6] Performance characteristics of a rapid immunochromatographic assay for detection of influenza virus in children during the 2003 to 2004 influenza season [J].

Cruz, AT ;

Cazacu, AC ;

McBride, LJ ;

Greer, JM ;

Demmier, GJ .

ANNALS OF EMERGENCY MEDICINE, 2006, 47 (03) :250-254

[7] Accuracy and interpretation of rapid influenza tests in children [J].

Grijalva, Carlos G. ;

Poehling, Katherine A. ;

Edwards, Kathryn M. ;

Weinberg, Geoffrey A. ;

Staat, Mary A. ;

Iwane, Marika K. ;

Schaffner, William ;

Griffin, Marie R. .

PEDIATRICS, 2007, 119 (01) :E6-E11

[8]

Hara Michimaru, 2004, Kansenshogaku Zasshi, V78, P935

[9]

Hara Michimaru, 2005, Kansenshogaku Zasshi, V79, P803

[10] Zanamivir for treatment of symptomatic influenza A and B infection in children five to twelve years of age: a randomized controlled trial [J].

Hedrick, JA ;

Barzilai, A ;

Behre, U ;

Henderson, FW ;

Hammond, J ;

Reilly, L ;

Keene, O .

PEDIATRIC INFECTIOUS DISEASE JOURNAL, 2000, 19 (05) :410-417

← 1 2 3 →