Development of a nitrocellulose-enzyme immunoassay for the detection of yellow-head virus from penaeid shrimp

被引:18
作者
Lu, Y [1 ]
Tapay, LM [1 ]
Loh, PC [1 ]
机构
[1] UNIV HAWAII,DEPT MICROBIOL,HONOLULU,HI 96822
关键词
D O I
10.1111/j.1365-2761.1996.tb00114.x
中图分类号
S9 [水产、渔业];
学科分类号
0908 ;
摘要
A nitrocellulose-enzyme immunoassay (NC-EIA) employing horseradish peroxidase (HRP) was developed for the detection of yellow-head virus in the tissues of infected penaeid shrimp. Two substrates, DAB (3,3 cent-diaminobenzidine tetrahydrochloride) and TMB (3,3 cent 5,5 cent-tetramethylbenzidine) for HRP were evaluated. The TMB was found to produce a more distinct and easily recognizable colour product and provided enhanced sensitivity (0 . 4 ng for TMB versus 0 . 8 ng viral protein For DAB). Under the present format, the NC-EIA was capable of detecting as little as 100 TCTD50 of virus in infected tissues. Uninfected tissue homogenates yielded no significant background colouration.
引用
收藏
页码:9 / 13
页数:5
相关论文
共 11 条
[1]  
BODE L, 1984, J VIROL METHODS, V8, P111, DOI 10.1016/0166-0934(84)90045-4
[2]   NON-OCCLUDED BACULO-LIKE VIRUS, THE CAUSATIVE AGENT OF YELLOW HEAD DISEASE IN THE BLACK TIGER SHRIMP (PENAEUS-MONODON) [J].
BOONYARATPALIN, S ;
SUPAMATTAYA, K ;
KASORNCHANDRA, J ;
DIREKBUSARACOM, S ;
AEKPANITHANPONG, U ;
CHANTANACHOOKLIN, C .
FISH PATHOLOGY, 1993, 28 (03) :103-109
[3]   HISTOLOGY AND ULTRASTRUCTURE REVEAL A NEW GRANULOSIS-LIKE VIRUS IN PENAEUS-MONODON AFFECTED BY YELLOW-HEAD DISEASE [J].
CHANTANACHOOKIN, C ;
BOONYARATPALIN, S ;
KASORNCHANDRA, J ;
DIREKBUSARAKOM, S ;
EKPANITHANPONG, U ;
SUPAMATAYA, K ;
SRIURAIRATANA, S ;
FLEGEL, TW .
DISEASES OF AQUATIC ORGANISMS, 1993, 17 (02) :145-157
[4]   A COMBINED DOT NITROCELLULOSE-ENZYME IMMUNOASSAY FOR ROTAVIRUS AND ADENOVIRUS [J].
GOUVEA, VS ;
DECASTRO, L ;
PEREIRA, HG .
JOURNAL OF VIROLOGICAL METHODS, 1987, 18 (01) :57-65
[5]   USE OF THE NITROCELLULOSE-ENZYME IMMUNOSORBENT-ASSAY FOR RAPID, SENSITIVE AND QUANTITATIVE DETECTION OF HUMAN ENTEROVIRUSES [J].
LOH, PC ;
DOW, MA ;
FUJIOKA, RS .
JOURNAL OF VIROLOGICAL METHODS, 1985, 12 (3-4) :225-234
[6]   INFECTION OF THE YELLOW HEAD BACULO-LIKE VIRUS (YBV) IN 2 SPECIES OF PENAEID SHRIMP, PENAEUS-STYLIROSTRIS (STIMPSON) AND PENAEUS-VANNAMEI (BOONE) [J].
LU, Y ;
TAPAY, LM ;
BROCK, JA ;
LOH, PC .
JOURNAL OF FISH DISEASES, 1994, 17 (06) :649-656
[7]   DEVELOPMENT OF A QUANTAL ASSAY IN PRIMARY SHRIMP CELL-CULTURE FOR YELLOW HEAD BACULOVIRUS (YBV) OF PENAEID SHRIMP [J].
LU, Y ;
TAPAY, LM ;
LOH, PC ;
BROCK, JA ;
GOSE, R .
JOURNAL OF VIROLOGICAL METHODS, 1995, 52 (1-2) :231-236
[8]  
LU Y, 1995, IN PRESS DIS AQUATIC
[9]   A NITROCELLULOSE-ENZYME IMMUNOASSAY METHOD FOR THE DETECTION OF HEPATITIS-A VIRUS [J].
NADALA, ECB ;
LOH, PC .
JOURNAL OF VIROLOGICAL METHODS, 1990, 28 (02) :155-163
[10]   A STREPTAVIDIN-BIOTIN-ENHANCED NITROCELLULOSE ENZYME-IMMUNOASSAY FOR THE DETECTION OF RHABDOVIRUS OF PENAEID SHRIMPS FROM INFECTED ANIMALS [J].
NADALA, ECB ;
LU, YA ;
LOH, PC ;
BROCK, JA .
JOURNAL OF VIROLOGICAL METHODS, 1992, 39 (1-2) :227-229