Electrophysiological effects of Bacillus sphaericus binary toxin on cultured mosquito cells

被引：29

作者：

Cokmus, C

Davidson, EW

Cooper, K

机构：

[1] ARIZONA STATE UNIV,DEPT ZOOL,TEMPE,AZ 85287

[2] ANKARA UNIV,DEPT BIOL,TR-06100 ANKARA,TURKEY

来源：

JOURNAL OF INVERTEBRATE PATHOLOGY | 1997年 / 69卷 / 03期

关键词：

Bacillus sphaericus; binary toxin; Culex quinquefasciatus; electrophysiological;

D O I：

10.1006/jipa.1997.4660

中图分类号：

Q95 [动物学];

学科分类号：

071002 ;

摘要：

The electrophysiological effects of both trypsin-activated native and 42- and 51-kDa cloned binary toxins of Bacillus sphaericus were investigated on cultured Culex quinquefasciatus cells using the patch-clamp technique. Rates of reduction in whole-cell membrane resistance were correlated with increasing native toxin concentration. The 42- or 51-kDa cloned toxin alone at 50 IJ;mu g/ml reduced the resistance. Electrophysiological effects occurred before any changes were visible by phase-contrast microscopy. (C) 1997 Academie Press.

引用

收藏

页码：197 / 204

页数：8

相关论文

共 31 条

[1] EFFECTS OF COMPONENTS OF THE BACILLUS-SPHAERICUS TOXIN ON MOSQUITO LARVAE AND MOSQUITO-DERIVED TISSUE CULTURE-GROWN CELLS [J].

BAUMANN, L ;

BAUMANN, P .

CURRENT MICROBIOLOGY, 1991, 23 (01) :51-57

[2] BACILLUS-SPHAERICUS AS A MOSQUITO PATHOGEN - PROPERTIES OF THE ORGANISM AND ITS TOXINS [J].

BAUMANN, P ;

CLARK, MA ;

BAUMANN, L ;

BROADWELL, AH .

MICROBIOLOGICAL REVIEWS, 1991, 55 (03) :425-436

[3] CONSTRUCTION BY SITE-DIRECTED MUTAGENESIS OF A 39-KILODALTON MOSQUITOCIDAL PROTEIN SIMILAR TO THE LARVA-PROCESSED TOXIN OF BACILLUS-SPHAERICUS 2362 [J].

BROADWELL, AH ;

CLARK, MA ;

BAUMANN, L ;

BAUMANN, P .

JOURNAL OF BACTERIOLOGY, 1990, 172 (07) :4032-4036

[4] THE 42-KILODALTON AND 51-KILODALTON MOSQUITOCIDAL PROTEINS OF BACILLUS-SPHAERICUS 2362 - CONSTRUCTION OF RECOMBINANTS WITH ENHANCED EXPRESSION AND INVIVO STUDIES OF PROCESSING AND TOXICITY [J].

BROADWELL, AH ;

BAUMANN, L ;

BAUMANN, P .

JOURNAL OF BACTERIOLOGY, 1990, 172 (05) :2217-2223

[5] LARVICIDAL PROPERTIES OF THE 42-KILODALTON AND 51-KILODALTON BACILLUS-SPHAERICUS PROTEINS EXPRESSED IN DIFFERENT BACTERIAL HOSTS - EVIDENCE FOR A BINARY TOXIN [J].

BROADWELL, AH ;

BAUMANN, L ;

BAUMANN, P .

CURRENT MICROBIOLOGY, 1990, 21 (06) :361-366

[6] Bacillus sphaericus toxins: Molecular biology and mode of action [J].

Charles, JF ;

NielsenLeRoux, C ;

Delecluse, A .

ANNUAL REVIEW OF ENTOMOLOGY, 1996, 41 :451-472

[7] DELETION ANALYSIS OF THE 51-KILODALTON PROTEIN OF THE BACILLUS-SPHAERICUS 2362 BINARY MOSQUITOCIDAL TOXIN - CONSTRUCTION OF DERIVATIVES EQUIVALENT TO THE LARVA-PROCESSED TOXIN [J].

CLARK, MA ;

BAUMANN, P .

JOURNAL OF BACTERIOLOGY, 1990, 172 (12) :6759-6763

[8]

Davidson E. W., 1989, Advances in Cell Culture, V7, P125

[9] BINDING OF THE BACILLUS-SPHAERICUS (EUBACTERIALES, BACILLACEAE) TOXIN TO MIDGUT CELLS OF MOSQUITO (DIPTERA, CULICIDAE) LARVAE, RELATIONSHIP TO HOST RANGE [J].

DAVIDSON, EW .

JOURNAL OF MEDICAL ENTOMOLOGY, 1988, 25 (03) :151-157

[10] INTERACTION OF THE BACILLUS-SPHAERICUS MOSQUITO LARVICIDAL PROTEINS [J].

DAVIDSON, EW ;

OEI, C ;

MEYER, M ;

BIEBER, AL ;

HINDLEY, J ;

BERRY, C .

CANADIAN JOURNAL OF MICROBIOLOGY, 1990, 36 (12) :870-878

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