Isolation and identification of melanin-concentrating hormone as the endogenous ligand of the SLC-1 receptor

被引：145

作者：

Shimomura, Y ^{[1
]}

Mori, M ^{[1
]}

Sugo, T ^{[1
]}

Ishibashi, Y ^{[1
]}

Abe, M ^{[1
]}

Kurokawa, T ^{[1
]}

Onda, H ^{[1
]}

Nishimura, O ^{[1
]}

Sumino, Y ^{[1
]}

Fujino, M ^{[1
]}

机构：

[1] Takeda Chem Ind Ltd, Pharmaceut Discovery Res Div, Discovery Res Labs 1, Ibaraki, Osaka 3004293, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1999年 / 261卷 / 03期

关键词：

D O I：

10.1006/bbrc.1999.1104

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Melanin-concentrating hormone (MCH), which is an orexigenic peptide, was isolated and identified as the endogenous ligand of the SLC-1 receptor. We established a CHO cell line expressing the rat SLC-1 receptor to search for its endogenous ligand. The extract of rat whole brain showed inhibition of intracellular forskolin-induced cAMP accumulation in rat SLC-1-expressing CHO cells and was purified. Using HPLC purification, we isolated and identified RICH as the endogenous ligand of the SLC-1 receptor. The authentic RICH demonstrated a dose-dependent inhibitory effect on cAMP accumulation in forskolin-stimulated rat and human SLC-l-expressing CHO cells with an EC50 value of 0.2 nM for both the rat and human SLC-1 receptors, This is the first description of the functional receptor for MCH. (C) 1999 Academic Press.

引用

页码：622 / 626

页数：5

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