Diverse bacteria associated with root nodules of spontaneous legumes in Tunisia and first report for nifH-like gene within the genera Microbacterium and Starkeya

被引:144
作者
Zakhia, Frederic
Jeder, Habib
Willems, Anne
Gillis, Monique
Dreyfus, Bernard
de Lajudie, Philippe [1 ]
机构
[1] Lab Symbioses Trop & Mediterraneennes, Montpellier, France
[2] Inst Reg Arides, Nahal Gabes, Tunisia
[3] Univ Ghent, Microbiol Lab, Ghent, Belgium
[4] USC II, INRA, LSTM, UMR113,IRD,CIRAD,AGRO,MUM,, F-34398 Montpellier 5, France
关键词
D O I
10.1007/s00248-006-9025-0
中图分类号
Q14 [生态学(生物生态学)];
学科分类号
071012 ; 0713 ;
摘要
We characterized 34 endophytic bacterial isolates associated to root nodules collected from spontaneous legumes in the arid zone of Tunisia by 16S rDNA polymerase chain reaction (PCR)-restriction fragment length polymorphism, whole cell protein sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), 16S rDNA and 16S-23S rDNA internal transcribed spacer sequencing. Phylogenetically, these isolates belong to the branches containing the genera Inquilinus, Bosea, Rhodopseudomonas, Paracraurococcus, Phyllobacterium, Ochrobactrum, Starkeya, Sphingomonas, Pseudomonas, Agromyces, Microbacterium, Ornithinicoccus, Bacillus, and Paenibacillus. These strains did not induce any nodule formation when inoculated on the wide host spectrum legume species M. atropurpureum (Siratro) and no nodA gene could be amplified by PCR. However, nifH sequences, most similar to those of Sinorhizobium meliloti, were detected within strains related to the genera Microbacterium, Agromyces, Starkeya and Phyllobacterium.
引用
收藏
页码:375 / 393
页数:19
相关论文
共 122 条
[1]   Rhizobium gallicum sp. nov. and Rhizobium giardinii sp. nov., from Phaseolus vulgaris nodules [J].
Amarger, N ;
Macheret, V ;
Laguerre, G .
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1997, 47 (04) :996-1006
[2]   RESPONSES OF BEAN TO ROOT COLONIZATION WITH PSEUDOMONAS-PUTIDA IN A HYDROPONIC SYSTEM [J].
ANDERSON, AJ ;
GUERRA, D .
PHYTOPATHOLOGY, 1985, 75 (09) :992-995
[3]   Phylogenetic affiliation of the pseudomonads based on 16S rRNA sequence [J].
Anzai, Y ;
Kim, H ;
Park, JY ;
Wakabayashi, H ;
Oyaizu, H .
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2000, 50 :1563-1589
[4]  
ASH C, 1993, ANTON LEEUW INT J G, V64, P253
[5]  
Bai YM, 2002, CAN J MICROBIOL, V48, P230, DOI [10.1139/w02-014, 10.1139/W02-014]
[6]  
BARANOVA N A, 1974, Mikrobiologiya, V43, P791
[7]  
BARRAQUIO W, 1988, NITROGEN FIXATION 10, P790
[8]  
BARTL S, 1994, BIOTECHNIQUES, V16, P246
[9]   Gamma proteobacteria can nodulate legumes of the genus Hedysarum [J].
Benhizia, Y ;
Benhizia, H ;
Benguedouar, A ;
Muresu, R ;
Giacomini, A ;
Squartini, A .
SYSTEMATIC AND APPLIED MICROBIOLOGY, 2004, 27 (04) :462-468
[10]   Surface colonization of lodgepole pine (Pinus contorta var. latifolia [Dougl. Engelm.]) roots by Pseudomonas fluorescens and Paenibacillus polymyxa under gnotobiotic conditions [J].
Bent, E ;
Breuil, C ;
Enebak, S ;
Chanway, CP .
PLANT AND SOIL, 2002, 241 (02) :187-196