Electrophoretic behavior of dyestuff-containing liposome in a capillary with original on-line chemiluminescence detection

An Eosin Y-containing liposome solution as a sample was for the first time migrated and detected by capillary zone electrophoresis with chemiluminescence detection using a peroxyoxalate system. Two peaks, as a typical example, were recorded on the electropherogram: the first one was due to Eosin Y entrapped in liposome; the second one was due to free Eosin Y in the bulk solution. The electropherogram, comprising two peaks, readily, rapidly, and sensitively offered various forms of information about the liposome, including the preparation course, stability, permeability, and surface charge. Furthermore, an Eosin Y-containing liposome solution gave a good linear relationship between the concentration and chemiluminescence intensity. Only the chemiluminescence detection was effective for examining the electrophoretic behavior of the Eosin Y-containing liposome in a capillary. Spectrophotometric and fluorescent detectors could not be utilized for detecting the liposome, due mainly to the low sensitivities.