Overexpression of a NAC transcription factor enhances rice drought and salt tolerance

被引：414

作者：

Zheng, Xingnan

Chen, Bo

Lu, Guojun

Han, Bin ^{[1
]}

机构：

[1] Chinese Acad Sci, Shanghai Inst Biol Sci, Natl Ctr Gene Res, Shanghai 200233, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2009年 / 379卷 / 04期

基金：

中国国家自然科学基金;

关键词：

Abiotic stress; Drought; Salt; Transcriptional activator; Oryza sativa; RESPONSIVE GENE-EXPRESSION; FUNCTIONAL-ANALYSIS; ORYZA-SATIVA; FREEZING TOLERANCE; SEQUENCE-ANALYSIS; ABIOTIC STRESS; ABSCISIC-ACID; ARABIDOPSIS; COLD; FAMILY;

D O I：

10.1016/j.bbrc.2008.12.163

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The plant-specific NAC (NAM, ATAF1/2, CUC2) transcription factors play diverse roles in plant development and stress responses. In this study, a rice NAC gene, ONAC045, was functionally characterized especially with regard to its role in abiotic stress resistance. Expression analysis revealed that ONAC045 was induced by drought, high salt, and low temperature stresses, and abscisic acid (ABA) treatment in leaves and roots. Transcriptional activation assay in yeast indicated that ONAC045 functioned as a transcriptional activator. Transient expression of GFP-ONAC045 in onion epidermal cells revealed that ONAC045 protein was localized in the nucleus. Transgenic rice plants overexpressing ONAC045 showed enhanced tolerance to drought and salt treatments. Two stress-responsive genes were upregulated in transgenic rice. Together, these results suggest that ONAC045 encodes a novel stress-responsive NAC transcription factor and is potential useful for engineering drought and salt tolerant rice. (C) 2009 Elsevier Inc. All rights reserved

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收藏

页码：985 / 989

页数：5

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