FISH landmarks for Aedes aegypti chromosomes

被引：26

作者：

Brown, SE ^{[1
]}

Knudson, DL ^{[1
]}

机构：

[1] COLORADO STATE UNIV,COLL AGR SCI,DEPT BIOAGR SCI & PEST MANAGEMENT,FT COLLINS,CO 80523

来源：

INSECT MOLECULAR BIOLOGY | 1997年 / 6卷 / 02期

关键词：

Aedes; aegypti; FISH; map; physical; chromosome;

D O I：

10.1111/j.1365-2583.1997.tb00088.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Aedes aegypti metaphase chromosome landmarks have been developed so that each chromosome of the haploid genome can be unambiguously identified and oriented by fluorescence in situ hybridization (FISH) and digital imaging microscopy. The FISH tags were derived from three cosmids that contain repetitive Ae. aegypti sequences and their unique FISH tagging characteristics are demonstrated. The sequence of the three chromosomal tags revealed that the chromosome 1 tag is an 18S fragment from the ribosomal cistron, and the other two chromosomal tags are repeats found in Ae. aegypfi with no apparent similarity to known sequences. A single plasmid that contains the three chromosome tag sequences has been constructed to simplify future FISH physical mapping.

引用

页码：197 / 202

页数：6

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