Replisome stalling and stabilization at CGG repeats, which are responsible for chromosomal fragility

被引：102

作者：

Voineagu, Irina ^{[1
,2
]}

Surka, Christine F. ^{[1
]}

Shishkin, Alexander A. ^{[1
]}

Krasilnikova, Maria M. ^{[3
]}

Mirkin, Sergei M. ^{[1
]}

机构：

[1] Tufts Univ, Dept Biol, Medford, MA 02155 USA

[2] Univ Illinois, Dept Biochem & Mol Genet, Chicago, IL 60607 USA

[3] Penn State Univ, Dept Biochem & Mol Biol, University Pk, PA 16802 USA

来源：

NATURE STRUCTURAL & MOLECULAR BIOLOGY | 2009年 / 16卷 / 02期

基金：

美国国家卫生研究院;

关键词：

SYNTHESIS IN-VITRO; DNA-REPLICATION; TRINUCLEOTIDE REPEATS; X-SYNDROME; S-PHASE; HUMAN-DISEASE; MRC1; FORM; INSTABILITY; TOF1;

D O I：

10.1038/nsmb.1527

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Expanded CGG repeats cause chromosomal fragility and hereditary neurological disorders in humans. Replication forks stall at CGG repeats in a length-dependent manner in primate cells and in yeast. Saccharomyces cerevisiae proteins Tof1 and Mrc1 facilitate replication fork progression through CGG repeats. Remarkably, the fork-stabilizing role of Mrc1 does not involve its checkpoint function. Thus, chromosomal fragility might occur when forks stalled at expanded CGG repeats escape the S-phase checkpoint.

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收藏

页码：226 / 228

页数：3

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