Genetic characterisation of CTX-M-15-producing Klebsiella pneumoniae and Escherichia coli strains isolated from stem cell transplant patients in Tunisia

被引:55
作者
Abbassi, Mohamed Salah [1 ]
Torres, Carmen [2 ]
Achour, Wafa [1 ]
Vinue, Laura [2 ]
Saenz, Yolanda [2 ]
Costa, Daniela [2 ]
Bouchami, Ons [1 ]
Ben Hassen, Assia [1 ]
机构
[1] Lab Natl Bone Marrow Transplantat Ctr, Tunis, Tunisia
[2] Univ La Rioja, Fac Ciencias, Area Bioquim & Biol Mol, Logrono 26006, Spain
关键词
ESBL; CTX-M-15; beta-lactamase; genetic environment; SHV-103; Klebsiella pneumoniae; Escherichia coli;
D O I
10.1016/j.ijantimicag.2008.04.009
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
Characterisation of extended-spectrum beta-lactamase ( ESBL) genes and their genetic environments as well as the presence of integrons were analysed in nine Klebsiella pneumoniae and two Escherichia coli ESBL-positive isolates recovered in the Centre of Bone Marrow Transplantation of Tunisia. All strains harboured the bla(CTX-M-15) gene and presented minimum inhibitory concentrations for cefotaxime and ceftazidime of 256-1024 mg L-1 and 16-512 mg L-1, respectively, and eight of them showed different pulsed-field gel electrophoresis patterns. The bla(OXA-1) and bla(TEM-1) genes were detected in eight and ten strains, respectively. In addition, bla(SHV-1), bla(SHV-11) and bla(SHV-27) were found in six, one and one K. pneumoniae strains, respectively. The new variant bla(SHV-103) was characterised in one K. pneumoniae strain. The intI1 gene was detected in eight K. pneumoniae strains and the dfrA5 + ereA2 and aadA gene cassettes were found in one and five strains, respectively. All strains harboured a 70 kb plasmid, and its transference in addition to bla(CTX-M-15), bla(TEM-1b) and bla(OXA-1) genes was demonstrated from three K. pneumoniae to E. coli. ISEcp1 and orf477 were located upstream and downstream, respectively, of the bla(CTX-M-15) gene in 10 strains. The occurrence of the bla(CTX-M-15) gene in unrelated strains might have originated from the dissemination of mobile genetic elements in which ISEcp1 may have played an important role. (C) 2008 Elsevier B. V. and the International Society of Chemotherapy. All rights reserved.
引用
收藏
页码:308 / 314
页数:7
相关论文
共 37 条
[1]  
BIRNBOIM HC, 1979, NUCLEIC ACIDS RES, V7, P1513
[2]   Complete nucleotide sequence of a 92-kilobase plasmid harboring the CTX-M-15 extended-spectrum beta-lactamase involved in an outbreak in long-term-care facilities in Toronto, Canada [J].
Boyd, DA ;
Tyler, S ;
Christianson, S ;
McGeer, A ;
Muller, MP ;
Willey, BM ;
Bryce, E ;
Gardam, M ;
Nordmann, P ;
Mulvey, MR .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2004, 48 (10) :3758-3764
[3]   Prevalence and spread of extended-spectrum β-lactamase-producing Enterobacteriaceae in Europe [J].
Canton, R. ;
Novais, A. ;
Valverde, A. ;
Machado, E. ;
Peixe, L. ;
Baquero, F. ;
Coque, T. M. .
CLINICAL MICROBIOLOGY AND INFECTION, 2008, 14 :144-153
[4]   The CTX-M β-lactamase pandemic [J].
Canton, Rafael ;
Coque, Teresa M. .
CURRENT OPINION IN MICROBIOLOGY, 2006, 9 (05) :466-475
[5]   Importance of integrons in the diffusion of resistance [J].
Carattoli, A .
VETERINARY RESEARCH, 2001, 32 (3-4) :243-259
[6]  
Clinical and laboratory Standards Institute, 2007, M100S17 CLSI
[7]   Dissemination of clonally related Escherichia coli strains expressing extended-spectrum β-lactamase CTX-M-15 [J].
Coque, Teresa M. ;
Novais, Angela ;
Carattoli, Alessandra ;
Poirel, Laurent ;
Pitout, Johann ;
Peixe, Luisa ;
Baquero, Fernando ;
Canton, Rafael ;
Nordmannj, Patrice .
EMERGING INFECTIOUS DISEASES, 2008, 14 (02) :195-200
[8]   Enterobacteriaceae [J].
Denton, Miles .
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS, 2007, 29 :S9-S22
[9]   DNA sequence analysis of the genetic environment of various blaCTX-M genes [J].
Eckert, C ;
Gautier, V ;
Arlet, G .
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2006, 57 (01) :14-23
[10]   Dissemination of CTX-M-type β-lactamases among clinical isolates of Enterobacteriaceae in Paris, France [J].
Eckert, C ;
Gautier, V ;
Saladin-Allard, M ;
Hidri, N ;
Verdet, C ;
Ould-Hocine, Z ;
Barnaud, G ;
Delisle, F ;
Rossier, A ;
Lambert, T ;
Philippon, A ;
Arlet, G .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2004, 48 (04) :1249-1255