This work describes preparation, characterization, and a moisture uptake study of silver nanoparticle-loaded calcium alginate films. The equilibrium uptake data was interpreted by various isotherm models. In the lower moisture content range of 0.02 to 0.06, the enthalpy of sorption (H) and differential entropy (S) were 25.25 to 4.82kJ/mol and 0.07521 to 0.01371kJ/mol/K, respectively. There was fair correlation between H and S values. Finally, the higher value of isokinetic temperature, TB (i.e., 336.77), as compared to the harmonic mean temperature, Tm (i.e., 302.05), indicated that moisture uptake mechanism was enthalpy driven.