Quercetin changes purinergic enzyme activities and oxidative profile in platelets of rats with hypothyroidism

被引：17

作者：

Baldissarelli, Jucimara ^{[1
]}

Santi, Adriana ^{[2
]}

Schmatz, Roberta ^{[3
]}

Zanini, Daniela ^{[1
]}

Cardoso, Andreia M. ^{[4
]}

Abadalla, Fatima H. ^{[5
]}

Thome, Gustavo R. ^{[1
]}

Murussi, Camila ^{[1
]}

Polachini, Carla R. N. ^{[1
]}

Delenogare, Diessica P. ^{[1
]}

Loro, Vania L. ^{[1
]}

Morsch, Vera M. ^{[1
]}

Schetinger, Maria R. C. ^{[1
]}

机构：

[1] Univ Fed Santa Maria, Ctr Ciencias Nat & Exatas, Programa Posgrad Ciencias Biol Bioquim Toxicol, Campus Univ, BR-97105900 Santa Maria, RS, Brazil

[2] Univ Fed Mato Grosso, Conselho Ensino & Pesquisa, Curso Med, Parque Sagrada Familia, Rondonopolis, MT, Brazil

[3] Inst Fed Educ Ciencia & Tecnol Rio Grande Sul, Campus Bento Goncalves, Bento Goncalves, RS, Brazil

[4] Univ Fed Fronteira Sul, Campus Chapeco, Chapeco, SC, Brazil

[5] Univ Luterana Brasil, Campus Carazinho, Canoas, RS, Brazil

来源：

BIOMEDICINE & PHARMACOTHERAPY | 2016年 / 84卷

关键词：

Hypothyroidism; Quercetin; Platelet aggregation; Ectonucleotidases; Oxidative stress; INDUCED DIABETIC-RATS; BLOOD-PLATELETS; 5'-NUCLEOTIDASE ACTIVITIES; ADENINE-NUCLEOTIDES; LIPID-PEROXIDATION; RISK-FACTORS; RED WINE; NTPDASE; ACETYLCHOLINESTERASE; ANTIOXIDANTS;

D O I：

10.1016/j.biopha.2016.10.109

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

100103 [病原生物学]; 100218 [急诊医学];

摘要：

Diseases related to thyroid hormones have been extensively studied because affect a large number of individuals, and these hormones participate in the regulation of the whole organism homeostasis. However, little is known about the involvement of purinergic signaling related to oxidative stress in hypothyroidism and possible therapeutic adjuncts for treatment of this disorder. Thus, the present study investigates the effects of quercetin on NTPDase, 5'-nucleotidase and adenosine deaminase activities, platelet aggregation and oxidative profile in platelets of rats with methimazole ( MMI)-induced hypothyroidism. Methimazole at a concentration of 20 mg/100 mL was administered for 90 days. From the second month the animals received quercetin 10 or 25 mg/kg for 60 days. Results showed that: Ecto50-nucleotidase activity decreased in methimazole/water group and the treatment with quercetin 25 mg/ kg decreased NTPDase, 5'-nucleotidase and adenosine deaminase activities. Moreover, platelet aggregation increased in methimazole/water group. Lipid peroxidation increased while superoxide dismutase and catalase activities decreased, but, interestingly, the treatment with quercetin reversed these changes. These results demonstrated that quercetin modulates adenine nucleotide hydrolysis decreasing the ADP formation and adenosine deamination. At the same time quercetin improves the oxidative profile, as well as reduces platelet aggregation, which together with the modulation in the nucleotides levels can contribute to the prevention of platelet disorders. (C) 2016 Elsevier Masson SAS. All rights reserved.

引用

页码：1849 / 1857

页数：9

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