Structure of the CRISPR Interference Complex CSM Reveals Key Similarities with Cascade

被引：155

作者：

Rouillon, Christophe ^{[1
]}

Zhou, Min ^{[4
]}

Zhang, Jing ^{[1
]}

Politis, Argyris ^{[4
]}

Beilsten-Edmands, Victoria ^{[4
]}

Cannone, Giuseppe ^{[2
,3
]}

Graham, Shirley ^{[1
]}

Robinson, Carol V. ^{[4
]}

Spagnolo, Laura ^{[2
,3
]}

White, Malcolm F. ^{[1
]}

机构：

[1] Univ St Andrews, St Andrews KY16 9ST, Fife, Scotland

[2] Univ Edinburgh, Inst Struct Mol Biol, Edinburgh EH9 3JR, Midlothian, Scotland

[3] Univ Edinburgh, Ctr Sci Extreme Condit, Edinburgh EH9 3JR, Midlothian, Scotland

[4] Univ Oxford, Dept Chem, Oxford OX1 3TA, England

来源：

MOLECULAR CELL | 2013年 / 52卷 / 01期

基金：

英国生物技术与生命科学研究理事会; 英国惠康基金;

关键词：

SULFOLOBUS-SOLFATARICUS; MACROMOLECULAR ASSEMBLIES; MASS-SPECTROMETRY; PROTEIN COMPLEXES; ESCHERICHIA-COLI; CAS SYSTEMS; TANDEM; IDENTIFICATION; STOICHIOMETRY; SUBCOMPLEX;

D O I：

10.1016/j.molcel.2013.08.020

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Clustered Regularly Interspaced Palindromic Repeats (CRISPR) system is an adaptive immune system in prokaryotes. Interference complexes encoded by CRISPR-associated (cas) genes utilize small RNAs for homology-directed detection and subsequent degradation of invading genetic elements, and they have been classified into three main types (I-III). Type III complexes share the Cas10 subunit but are subclassifed as type IIIA (CSM) and type IIIB (CMR), depending on their specificity for DNA or RNA targets, respectively. The role of CSM in limiting the spread of conjugative plasmids in Staphylococcus epidermidis was first described in 2008. Here, we report a detailed investigation of the composition and structure of the CSM complex from the archaeon Sulfolobus solfataricus, using a combination of electron microscopy, mass spectrometry, and deep sequencing. This reveals a three-dimensional model for the CSM complex that includes a helical component strikingly reminiscent of the backbone structure of the type I (Cascade) family.

引用

页码：124 / 134

页数：11

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