The HopPtoF locus of Pseudomonas syringae pv. tomato DC3000 encodes a type III chaperone and a cognate effector

被引：34

作者：

Shan, LB

Oh, HS

Chen, JF

Guo, M

Zhou, JM

Alfano, JR

Collmer, A

Jia, X

Tang, XY

机构：

[1] Kansas State Univ, Dept Plant Pathol, Manhattan, KS 66506 USA

[2] Acad Sinica, Inst Genet & Dev Biol, Beijing 100101, Peoples R China

[3] Univ Nebraska, Dept Plant Pathol, Lincoln, NE 68588 USA

[4] Univ Nebraska, Plant Sci Initiat, Lincoln, NE 68588 USA

[5] Cornell Univ, Dept Plant Pathol, Ithaca, NY 14853 USA

来源：

MOLECULAR PLANT-MICROBE INTERACTIONS | 2004年 / 17卷 / 05期

关键词：

D O I：

10.1094/MPMI.2004.17.5.447

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Type III secretion systems are highly conserved among gram-negative plant and animal pathogenic bacteria. Through the type III secretion system, bacteria inject a number of virulence proteins into the host cells. Analysis of the whole genome sequence of Pseudomonas syringae pv. tomato DC3000 strain identified a locus, named HopPtoF, that is homologous to the avirulence gene locus avrPphF in R syringae pv. phaseolicola. The HopPtoF locus harbors two genes, ShcF(Pto) and HopF(Pto) that are preceded by a single hrp box promoter. We present evidence here to show that ShcF(Pto) and HopF(Pto) encode a type III chaperone and a cognate effector, respectively. ShcF(Pto) interacts with and stabilizes the HopF(Pto) protein in the bacterial cell. Translation of HopF(Pto) starts at a rare initiation codon ATA that limits the synthesis of the HopF(Pto) protein to a low level in bacterial cells.

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页码：447 / 455

页数：9

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