Secretion of the Escherichia coli K-12 sheA hemolysin is independent of its cytolytic activity

被引：18

作者：

del Castillo, FJ ^{[1
]}

Moreno, F ^{[1
]}

del Castillo, I ^{[1
]}

机构：

[1] Hosp Ramon y Cajal, Mol Genet Unit, E-28034 Madrid, Spain

来源：

FEMS MICROBIOLOGY LETTERS | 2001年 / 204卷 / 02期

关键词：

SheA hemolysin; bacterial protein secretion; beta-lactamase gene fusion; outer membrane vesicle;

D O I：

10.1016/S0378-1097(01)00413-X

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The Escherichia coli K-12 sheA gene encodes a pore-forming hemolysin that is secreted to the medium by a hitherto unidentified mechanism. To study SheA secretion, we constructed fusions between SheA and the mature form of the periplasmic enzyme beta -lactamase, and performed site-directed mutagenesis on these constructs. The SheA-Bla and Bla-SheA hybrid proteins displayed hemolytic activity and were efficiently exported to the extracellular medium. Our results with mutant hybrid proteins show that secretion of SheA is independent of its cytolytic activity, that secretion is paralleled by a transient leakage of periplasmic contents to the extracellular medium, and that deletion of the 11 C-terminal residues, of SheA has no effect on its secretion and cytolytic activity. (C) 2001 Federation of European Microbiological Societies, Published by Elsevier Science BN. All rights reserved.

引用

页码：281 / 285

页数：5

共 22 条

[1] PARTIAL AMINO-ACID SEQUENCE OF PENICILLINASE CODED BY ESCHERICHIA-COLI PLASMID R6K [J].

AMBLER, RP ;

SCOTT, GK .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1978, 75 (08) :3732-3736

[2] Structures of gram-negative cell walls and their derived membrane vesicles [J].

Beveridge, TJ .

JOURNAL OF BACTERIOLOGY, 1999, 181 (16) :4725-4733

[3] BETA-LACTAMASE AS A PROBE OF MEMBRANE-PROTEIN ASSEMBLY AND PROTEIN EXPORT [J].

BROOMESMITH, JK ;

TADAYYON, M ;

ZHANG, Y .

MOLECULAR MICROBIOLOGY, 1990, 4 (10) :1637-1644

[4] Characterization of the genes encoding the SheA haemolysin in Escherichia coli O157:H7 and Shigella flexneri 2a [J].

del Castillo, FJ ;

Moreno, F ;

del Castillo, I .

RESEARCH IN MICROBIOLOGY, 2000, 151 (03) :229-230

[5] The Escherichia coli K-12 sheA gene encodes a 34-kDa secreted haemolysin [J].

delCastillo, FJ ;

Leal, SC ;

Moreno, F ;

delCastillo, I .

MOLECULAR MICROBIOLOGY, 1997, 25 (01) :107-115

[6] Regulation of the Escherichia coli sheA gene and characterization of its encoded hemolytic activity [J].

Fernández, SV ;

Xing, J ;

Kapur, V ;

Libby, SJ ;

Barletta, RG ;

Moxley, RA .

FEMS MICROBIOLOGY LETTERS, 1998, 168 (01) :85-90

[7]

HELMSLEY A, 1989, NUCLEIC ACIDS RES, V17, P6545

[8]

Kolling GL, 1999, APPL ENVIRON MICROB, V65, P1843

[9] THE NORMALLY PERIPLASMIC ENZYME BETA-LACTAMASE IS SPECIFICALLY AND EFFICIENTLY TRANSLOCATED THROUGH THE ESCHERICHIA-COLI OUTER-MEMBRANE WHEN IT IS FUSED TO THE CELL-SURFACE ENZYME PULLULANASE [J].

KORNACKER, MG ;

PUGSLEY, AP .

MOLECULAR MICROBIOLOGY, 1990, 4 (07) :1101-1109

[10] Cytocidal and apoptotic effects of the ClyA protein from Escherichia coli on primary and cultured monocytes and macrophages [J].

Lai, XH ;

Arencibia, I ;

Johansson, A ;

Wai, SN ;

Oscarsson, J ;

Kalfas, S ;

Sundqvist, KG ;

Mizunoe, Y ;

Sjöstedt, A ;

Uhlin, BE .

INFECTION AND IMMUNITY, 2000, 68 (07) :4363-4367

← 1 2 3 →