Preparation of a microperoxidase and ferrocene-immobilized polyion complex membrane for the detection of hydrogen peroxide

被引：43

作者：

Yabuki, S ^{[1
]}

Mizutani, F ^{[1
]}

Hirata, Y ^{[1
]}

机构：

[1] Natl Inst Biosci & Human Technol, Tsukuba, Ibaraki 3058566, Japan

来源：

JOURNAL OF ELECTROANALYTICAL CHEMISTRY | 1999年 / 468卷 / 01期

关键词：

amperometric sensor; biosensors; membrane-covered electrode; hydrogen peroxide; ferrocene;

D O I：

10.1016/S0022-0728(99)00088-1

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A polyion complex membrane containing physically entrapped microperoxidase and ferrocene was prepared on a glassy carbon electrode. A reductive current response for H2O2 was obtained under the application of 0.0 V versus Ag \ AgCl. The current response was proportional to the H2O2 concentration up to 20 mu M, and the lower detection limit was 0.5 mu M (S/N = 5). The interference current from L-ascorbate was ca. 45% of the current for the same concentration of H2O2. The electrode could be used for 10 days. The electrode was applied as the transducer of an oxidase-based sensor such as a glucose oxidase-based one: the glucose-sensing electrode prepared showed high sensitivity (lower detection limit, 5 mu M). (C) 1999 Elsevier Science S.A. All rights reserved.

引用

页码：117 / 120

页数：4

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