Overexpression of lipase A and B of Geotrichum candidum in Pichia pastoris: High-level production and some properties of functional expressed lipase B

被引：31

作者：

Catoni, E ^{[1
]}

SchmidtDannert, C ^{[1
]}

Brocca, S ^{[1
]}

Schmid, RD ^{[1
]}

机构：

[1] UNIV STUTTGART, INST TECH BIOCHEM, D-70569 STUTTGART, GERMANY

来源：

BIOTECHNOLOGY TECHNIQUES | 1997年 / 11卷 / 09期

关键词：

D O I：

10.1023/A:1018419729009

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Lipase A and B from Geotrichum candidum CMICC 335426 were functionally expressed and secreted in Pichia pastoris. Lipase B exhibits a unique substrate specificity for long-chain cis-Delta 9 unsaturated triacylglycerols. Thus, using different cultivation conditions, the production of recombinant lipase B was optimised, yielding 200 000 Units per liter culture. After overexpression lipase B was purified by diafiltration (372 U/mg). The investigated enzyme properties were comparable to those reported for the native enzyme.

引用

页码：689 / 695

页数：7

共 15 条

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