Role of calmodulin in the modulation of the MAPK signalling pathway and the transactivation of epidermal growth factor receptor mediated by PKC

被引：34

作者：

Tebar, F

Lladó, A

Enrich, C

机构：

[1] Departament de Biologia Cellular, Inst. d'Investigacions Biomediques August Pi i Sunyer, Univ. de Barcelona, 08036 Barcelona

来源：

FEBS LETTERS | 2002年 / 517卷 / 1-3期

关键词：

epidermal growth factor receptor; calmodulin; protein kinase C; transactivation; calmodulin antagonist; mitogen-activated protein kinase; shedding;

D O I：

10.1016/S0014-5793(02)02624-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have recently shown that calmodulin (CaM) regulates the trafficking of epidermal growth factor receptor (EGFR) as well as the mitogen-activated protein kinase (MAPK) signalling pathway. However, the overall regulation of the MAPK pathway is achieved through a complex interplay of other several upstream effectors including G-proteins, EGF, EGFR, protein kinase C (PKC), phosphatidylinositol-3-kinase and CaM. In order to understand the role of CaM in the PKC-mediated transactivation of EGFR we have analysed the effect of a CaM antagonist, N-(4-aminobutyl)-5-chloro-2-naphthalenesulfonamide, on the 12-O-tetradecanoylphorbol-13-acetate-mediated activation of EGFR and the subsequent MAPK activation. The results show that CaM interferes with MAPK activation and the transactivation of EGFR mediated by PKC. (C) 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：206 / 210

页数：5

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