Residual oil fly ash exposure enhances allergic sensitization to house dust mite

被引:60
作者
Lambert, AL [1 ]
Dong, WM
Winsett, DW
Selgrade, MK
Gilmour, MI
机构
[1] Univ N Carolina, Curriculum Toxicol, Chapel Hill, NC 27599 USA
[2] US EPA, Natl Hlth & Environm Effects Res Lab, Res Triangle Pk, NC 27711 USA
关键词
particulate matter; house dust mite; allergy; rat; lung; IgE; adjuvant; IL-10;
D O I
10.1006/taap.1999.8709
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Epidemiological studies have shown an association between elevated levels of particulate matter air pollution and increased morbidity and hospital visits in asthmatics. Residual oil fly ash (ROFA) is a primary combustion particle containing sulfate and metals such as vanadium, nickel, and iron. In this study the effect of ROFA on sensitization to house dust mite (HDM) was examined in a Brown Norway rat model of pulmonary allergy. Rats were instilled via the trachea with 200 or 1000 mu g ROFA 3 days prior to local sensitization with 10 mu g HDM and were challenged with 10 mu g HDM 14 days later. Immunological endpoints were examined at 2, 7, and 14 days after sensitization and at 2 and 7 days after challenge (16 and 21 days post-sensitization, respectively). Antigen-specific immunoglobulin E and associated immediate bronchoconstriction responses to antigen challenge were increased in the ROFA-treated groups compared with the HDM control group. Lymphocyte proliferation to antigen was enhanced at Days 7 and 21 in the bronchial lymphocytes of ROFA-treated groups, Bronchoalveolar lavage fluid (BALF) eosinophil numbers and lactate dehydrogenase were significantly increased in the 1000 mu g ROFA group at Days 2 and 16, BALE total proteins were elevated at Days 2 and 7 in both ROFA-treated groups, and BALF interleukin (IL)-10 was elevated in the 1000 mu g ROFA group at Day 2. These results suggest that ROFA has an adjuvant effect on sensitization to HDM. (C) 1999 Academic Press.
引用
收藏
页码:269 / 277
页数:9
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