Inhibition of T cell receptor-coreceptor interactions by antagonist ligands visualized by live FRET imaging of the T-hybridoma immunological synapse

被引：115

作者：

Zal, T ^{[1
]}

Zal, MA ^{[1
]}

Gascoigne, NRJ ^{[1
]}

机构：

[1] Scripps Res Inst, Dept Immunol, La Jolla, CA 92037 USA

来源：

IMMUNITY | 2002年 / 16卷 / 04期

关键词：

D O I：

10.1016/S1074-7613(02)00301-1

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The diverse effects of TCR agonists and antagonists on T cell activation are believed to be modified by the differential recruitment of CD4 or CD8 coreceptors to the TCR-MHCp complex. We used three-dimensional live cell imaging of fluorescence resonance energy transfer (FRET) between CD3zeta and CD4 fused to variants of the green fluorescent protein to investigate TCR-CD4 interactions during T cell activation, We demonstrate that recognition of agonist MHCp complexes triggers intermolecular interaction between CD4 and TCR, detectable across the T-hybridoma-APC contact area. This interaction is blocked by the presence of antagonist ligands without decreasing the recruitment of and CD4 or preventing their partial colocalization in the immunological synapse.

引用

页码：521 / 534

页数：14

共 35 条

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