Low mRNA levels of RGS4 splice variants in Alzheimer's disease: Association between a rare haplotype and decreased mRNA expression

被引：30

作者：

Emilsson, L ^{[1
]}

Saetre, P ^{[1
]}

Jazin, E ^{[1
]}

机构：

[1] Uppsala Univ, Dept Evolut Genom & Systemat, S-75236 Uppsala, Sweden

来源：

SYNAPSE | 2006年 / 59卷 / 03期

关键词：

neurodegeneration; RGS splice variants; SNP; real-time RT-PCR;

D O I：

10.1002/SYN.20226

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Regulator of G-protein signaling 4 (RGS4) showed decreased mRNA levels in Alzheimer's disease in a large collection of human brain autopsies from prefrontal cortex. The expression levels of three RGS4 splice variants were examined in the same samples, and the association between RGS4 gene expression and/or the disease with single nucleotide polymorphisms located in this gene was explored. We show that all splice variants are down-regulated in patients. We also demonstrate that one rare haplotype (ATAG) is associated with decreased mRNA levels in both cases and controls. Our results suggest that an altered regulation in transcription initiation may be an important mechanism for low RGS4 protein levels in Alzeimer's disease.

引用

页码：173 / 176

页数：4

共 18 条

[1] The quantification of gene expression in an animal model of brain ischaemia using TaqMan™ real-time RT-PCR [J].

Bond, BC ;

Virley, DJ ;

Cairns, NJ ;

Hunter, AJ ;

Moore, GBT ;

Moss, SJ ;

Mudge, AW ;

Walsh, FS ;

Jazin, E ;

Preece, P .

MOLECULAR BRAIN RESEARCH, 2002, 106 (1-2) :101-116

[2] High-resolution quantification of specific mRNA levels in human brain autopsies and biopsies [J].

Castensson, A ;

Emilsson, L ;

Preece, P ;

Jazin, E .

GENOME RESEARCH, 2000, 10 (08) :1219-1229

[3] Regulator of G-protein signaling 4 (RGS4) gene is associated with schizophrenia in Irish high density families [J].

Chen, XN ;

Dunham, C ;

Kendler, S ;

Wang, X ;

O'Neill, FA ;

Walsh, D ;

Kendler, KS .

AMERICAN JOURNAL OF MEDICAL GENETICS PART B-NEUROPSYCHIATRIC GENETICS, 2004, 129B (01) :23-26

[4] Association and linkage analyses of RGS4 polymorphisms in schizophrenia [J].

Chowdari, KV ;

Mirnics, K ;

Semwal, P ;

Wood, J ;

Lawrence, E ;

Bhatia, T ;

Deshpande, SN ;

K, TB ;

Ferrell, RE ;

Middleton, FA ;

Devlin, B ;

Levitt, P ;

Lewis, DA ;

Nimgaonkar, VL .

HUMAN MOLECULAR GENETICS, 2002, 11 (12) :1373-1380

[5]

Cowburn RF, 2001, BIOCHEM SOC SYMP, V67, P163

[6] The regulator of G protein signaling family [J].

De Vries, L ;

Zheng, B ;

Fischer, T ;

Elenko, E ;

Farquhar, MG .

ANNUAL REVIEW OF PHARMACOLOGY AND TOXICOLOGY, 2000, 40 :235-271

[7] Pedigree disequilibrium tests for multilocus haplotypes [J].

Dudbridge, F .

GENETIC EPIDEMIOLOGY, 2003, 25 (02) :115-121

[8] Increased monoamine oxidase messenger RNA expression levels in frontal cortex of Alzheimer's disease patients [J].

Emilsson, L ;

Saetre, P ;

Balciuniene, J ;

Castensson, A ;

Cairns, N ;

Jazin, EE .

NEUROSCIENCE LETTERS, 2002, 326 (01) :56-60

[9]

EMILSSON L, 2005, NEUROBIOL DIS

[10] Confirming RGS4 as a susceptibility gene for schizophrenia [J].

Morris, DW ;

Rodgers, A ;

McGhee, KA ;

Schwaiger, S ;

Scully, P ;

Quinn, J ;

Meagher, D ;

Waddington, JL ;

Gill, M ;

Corvin, AP .

AMERICAN JOURNAL OF MEDICAL GENETICS PART B-NEUROPSYCHIATRIC GENETICS, 2004, 125B (01) :50-53

← 1 2 →